Department of Botany, University of Science & Technology Bannu, Bannu, KPK, Pakistan.
Faculty of Medicine, Department of Pharmacology, King Abdulaziz University, Jeddah, Saudi Arabia.
PLoS One. 2024 Nov 15;19(11):e0305751. doi: 10.1371/journal.pone.0305751. eCollection 2024.
Berula erecta L. is traditionally used for the treatment of various human ailments. The present project was arranged to study the antioxidant and anti-Parkinson efficacy of B. erecta extracts against rotenone-induced Parkinson diseases in rats. Fine powder of the plant was extracted with methanol and then fractionated through various solvents with increasing order of polarity. Phytochemical screenings were done using standard protocols and High-performance liquid chromatography (HPLC) while in-vitro antioxidant activities of plant fractions were evaluated using different free radicals. In-vivo anti-Parkinson and oxidative dysfunction experiments were conducted in rats. Results revealed that various fractions possessed flavonoids, alkaloids, terpenoids saponins, tannin, anthraquinon, and phlobatanine, while terpeniods and alkaloids were absent in aqueous fraction. Chromatographic analysis of methanol fraction showed the presence of various bioactive compounds viz., vitexin, orientin, rutin, catechin and myricetin. In-vitro antioxidant activities of various fractions of Berula erecta (B.erecta) showed that methanol fraction has remarkable scavenging efficacy of 2,2-Diphenyl-1-picrylhydrazyl (DPPH), beta carotene, and superoxide free radicals followed by chloroform fraction. Free radicals produced by 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS), Hydrogen peroxide (H2O2), and hydroxyl free radicals were considerably scavenged by methanol fraction followed by ethyl acetate fractions. In-vivo study of animal model showed that methanol fraction has significant recovery effects at behavioural, physiological and biochemical level against rotenone induced Parkinson disease. B.erecta has significantly improved rotenone-induced motor and nonmotor deficits (depression and cognitive impairments), increased antioxidant enzyme activity, and reduced neurotransmitter changes. It has been concluded from the present data that B.erecta enhances neurotransmitter levels by alleviating oxidative stress and antioxidant enzyme activity, hence improving motor activity, cognitive functioning, and decreasing depressed behavior. These data suggest that B. erecta may be a promising medicinal agent for reducing the risk and progression of Parkinson's disease.
直立委陵菜传统上用于治疗各种人类疾病。本项目旨在研究直立委陵菜提取物对鱼藤酮诱导的大鼠帕金森病的抗氧化和抗帕金森疗效。植物的细粉用甲醇提取，然后用不同极性递增的溶剂进行分级。使用标准方案和高效液相色谱（HPLC）进行植物成分的生化筛选，同时使用不同的自由基评估植物馏分的体外抗氧化活性。在大鼠中进行体内抗帕金森和氧化功能障碍实验。结果表明，各种馏分含有类黄酮、生物碱、萜类、皂苷、单宁、蒽醌和菲烷，而水馏分中则没有萜类和生物碱。甲醇馏分的色谱分析表明存在各种生物活性化合物，如牡荆素、橙皮苷、芦丁、儿茶素和杨梅素。直立委陵菜（B.erecta）的各种馏分的体外抗氧化活性表明，甲醇馏分对 2,2-二苯基-1-苦基肼（DPPH）、β-胡萝卜素和超氧自由基具有显著的清除作用，其次是氯仿馏分。ABTS、H2O2 和羟基自由基产生的自由基被甲醇馏分显著清除，其次是乙酸乙酯馏分。动物模型的体内研究表明，甲醇馏分在行为、生理和生化水平上对鱼藤酮诱导的帕金森病具有显著的恢复作用。B.erecta 显著改善了鱼藤酮诱导的运动和非运动缺陷（抑郁和认知障碍），增加了抗氧化酶活性，减少了神经递质变化。从目前的数据可以得出结论，B.erecta 通过减轻氧化应激和抗氧化酶活性来提高神经递质水平，从而改善运动活动、认知功能和减少抑郁行为。这些数据表明，B. erecta 可能是一种有前途的药物，可降低帕金森病的风险和进展。
