Department of Biotechnology, Chandigarh University, Gharuan, Mohali, 140413, Punjab, India.
University Centre for Research and Development, Chandigarh University, Gharuan, Mohali, 140413, Punjab, India.
Mikrochim Acta. 2024 Nov 16;191(12):745. doi: 10.1007/s00604-024-06838-8.
A fluorescence (FL)-based nanosensor has been devised for creatinine (CR) detection in human urine specimens. The proposed nanosensor utilized a nanocomposite (NC) of carbon dots (CDs) and graphene oxide (GO). The formation of CDs/GO NC reduced the CD FL emission (λ = 390 nm, λ = 461 nm) by ~ 75%. With the introduction of CR to the NC, the CD emission intensity was reinstated by approximately 70%. The linear detection range for CR was 10 to 0.1 mg dL (R = 0.998), with a limit of detection of 4.3 × 10 mg dL. Additionally, CDs/GO NC exhibited outstanding consistency and specificity in recognizing CR within urine specimens from both healthy individuals and patients suffering from chronic kidney disease (CKD). The Bland-Altman assessment (utilizing 25 human urine specimens) displayed remarkable consensus (R = 0.995) among the FL approach and the benchmark Jaffe technique. This observation indicates the hands-on usefulness of the nanosensor for identifying CR in biological specimens.
一种基于荧光（FL）的纳米传感器已被设计用于检测人尿液样本中的肌酸酐（CR）。所提出的纳米传感器利用了碳点（CD）和氧化石墨烯（GO）的纳米复合材料（NC）。CD/GO NC 的形成使 CD 的 FL 发射（λ=390nm，λ=461nm）降低了约 75%。当将 CR 引入 NC 时，CD 的发射强度恢复了约 70%。CR 的线性检测范围为 10 至 0.1mg/dL（R=0.998），检测限为 4.3×10mg/dL。此外，CD/GO NC 在识别来自健康个体和慢性肾病（CKD）患者的尿液样本中的 CR 方面表现出出色的一致性和特异性。（利用 25 个人类尿液样本）Bland-Altman 评估显示，FL 方法和基准 Jaffe 技术之间具有显著的一致性（R=0.995）。这一观察结果表明，该纳米传感器在识别生物样本中的 CR 方面具有实际应用价值。
