Breedveld F C, Lafeber G J, van den Barselaar M T, van Dissel J T, Leijh P C
Arthritis Rheum. 1986 Feb;29(2):166-73. doi: 10.1002/art.1780290203.
The phagocytosis and intracellular killing by synovial fluid (SF) polymorphonuclear cells (PMN) of 10 patients with rheumatoid arthritis was studied. PMN phagocytosis was assessed by morphologic and microbiologic methods and intracellular killing was measured independently of continuous phagocytosis of Staphylococcus aureus. Phagocytosis of S aureus by SF PMN or peripheral blood (PB) PMN was as effective in the presence of synovial fluid as in the presence of serum. On average, SF PMN ingested S aureus opsonized with synovial fluid and serum more efficiently than patient or donor PB PMN did. Enhanced ingestion of S aureus was associated with increased expression of C3 receptors on the membrane of SF PMN. In the presence of heat-inactivated synovial fluid, the capacity of SF PMN to ingest S aureus was greater than that of patient or donor PB PMN. Under these conditions, phagocytic activity was correlated with Fc receptor expression. SF PMN was found to be as active in killing S aureus as PB PMN from healthy donors.
对10例类风湿关节炎患者滑液（SF）中的多形核白细胞（PMN）的吞噬作用和细胞内杀菌作用进行了研究。通过形态学和微生物学方法评估PMN的吞噬作用，并独立于金黄色葡萄球菌的持续吞噬作用来测定细胞内杀菌作用。在滑液存在的情况下，SF PMN或外周血（PB）PMN对金黄色葡萄球菌的吞噬作用与在血清存在时一样有效。平均而言，SF PMN比患者或供体PB PMN更有效地摄取经滑液和血清调理的金黄色葡萄球菌。金黄色葡萄球菌摄取量的增加与SF PMN膜上C3受体表达的增加有关。在热灭活滑液存在的情况下，SF PMN摄取金黄色葡萄球菌的能力大于患者或供体PB PMN。在这些条件下，吞噬活性与Fc受体表达相关。发现SF PMN在杀死金黄色葡萄球菌方面与健康供体的PB PMN一样活跃。
