细菌视紫红质在低pH值下质子摄取和释放的顺序。
Order of proton uptake and release by bacteriorhodopsin at low pH.
作者信息
Mitchell D, Rayfield G W
出版信息
Biophys J. 1986 Feb;49(2):563-6. doi: 10.1016/S0006-3495(86)83666-9.
Abstract
The order of proton uptake and release in an aqueous suspension of purple membrane in response to a light flash has been investigated at lowered pH. pH indicator dyes and a flash spectrophotometer were used for the study. At pH 6.6 it was found that the release of protons from the purple membrane precedes uptake, as reported by other investigators. At pH 5.9, 4.9, and 4.1 it was also found that release precedes uptake. These results are not in agreement with those of previous investigators.
摘要
在较低pH值下，研究了紫膜水悬浮液中质子响应光脉冲吸收和释放的顺序。使用pH指示剂染料和闪光分光光度计进行该研究。在pH 6.6时，发现如其他研究者所报道的那样，紫膜中质子的释放先于吸收。在pH 5.9、4.9和4.1时，也发现释放先于吸收。这些结果与先前研究者的结果不一致。
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本文引用的文献
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Infrared evidence that the Schiff base of bacteriorhodopsin is protonated: bR570 and K intermediates.细菌视紫红质席夫碱质子化的红外证据：bR570和K中间体。
Proc Natl Acad Sci U S A. 1982 Jul;79(13):4045-9. doi: 10.1073/pnas.79.13.4045.
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Nonionic detergent effects on spectroscopic characteristics and the photocycle of bacteriorhodopsin in purple membranes.非离子去污剂对紫膜中细菌视紫红质光谱特性及光循环的影响。
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Proc Natl Acad Sci U S A. 1983 Jan;80(1):178-80. doi: 10.1073/pnas.80.1.178.
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Bacteriorhodopsin and related pigments of halobacteria.嗜盐菌的细菌视紫红质及相关色素。
Annu Rev Biochem. 1982;51:587-616. doi: 10.1146/annurev.bi.51.070182.003103.
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