细菌视紫红质中pH诱导光谱变化的动力学
Dynamics of pH-induced spectral changes in bacteriorhodopsin.
作者信息
Druckmann S, Samuni A, Ottolenghi M
出版信息
Biophys J. 1979 Apr;26(1):143-5. doi: 10.1016/S0006-3495(79)85241-8.
The kinetics of the spectral shift induced in bacteriorhodopsin by low pH are investigated by using the rapid-mixing, stopped-flow technique. The generation of the acid form of the chromophore (A605) occurs in two distinct steps: a fast process (t1/2I = 21 +/- 4 ms) is followed by a much slower reaction (t1/2II = 6 +/- 2 s). The observations are interpreted in terms of neutralization of an acid group in the neighborhood of the retinyl chromophore, the double-staged kinetics being attributed to cooperative effects between chromophores. The method may serve as a tool for studying the kinetics of proton migration across the purple membrane.
利用快速混合、停流技术研究了低pH值诱导细菌视紫红质光谱位移的动力学。发色团酸性形式（A605）的生成分为两个不同的步骤：一个快速过程（t1/2I = 21 +/- 4毫秒）之后是一个慢得多的反应（t1/2II = 6 +/- 2秒）。这些观察结果被解释为视黄基发色团附近一个酸性基团的中和，双阶段动力学归因于发色团之间的协同效应。该方法可作为研究质子跨紫色膜迁移动力学的工具。
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