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基于结构的天然化合物预防皮肤衰老的虚拟筛选：表没食子儿茶素没食子酸酯在蛋白激酶Cα特异性抑制紫外线诱导光老化中的作用

Structure-based virtual screening of natural compounds in preventing skin senescence: The role of epigallocatechin gallate in protein kinase C alpha-specific inhibition against UV-induced photoaging.

作者信息

Cho Cheol Hyeon, Sim Woo-Jin, Cho Nam-Chul, Lim Wonchul, Lim Tae-Gyu

机构信息

Department of Food Science & Biotechnology, Sejong University, Seoul 05006, Republic of Korea.

Korea Chemical Bank, Korea Research Institute of Chemical Technology (KRICT), Daejeon, 34114, Republic of Korea.

出版信息

Heliyon. 2024 Oct 30;10(21):e39933. doi: 10.1016/j.heliyon.2024.e39933. eCollection 2024 Nov 15.

DOI:10.1016/j.heliyon.2024.e39933
PMID:39553571
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11567019/
Abstract

This study combines high-throughput screening and virtual molecular docking to identify natural compounds targeting PKC in skin aging. Go 6983, a PKC inhibitor, showed potent suppression of MMP-1 transcription. EGCG was one of the candidates that showed it could significantly lower UVB-induced MMP-1 expression in HaCaT cells, and it had a strong affinity for PKCα. Interestingly, EGCG is exclusively bound to PKCα, not the δ and ζ isoforms. Blocking PKCα did not elevate UVB-induced MMP-1 expression in HaCaT cells. In a model of human skin, EGCG stopped collagen breakdown and changes in epidermal thickness that were caused by UV light from the sun. This suggests that EGCG could be useful in dermatology and drug development. These findings highlight the role of structure-based screening in identifying candidate compounds with applications in the cosmetic, dermatological, preventive health, and pharmaceutical fields.

摘要

本研究结合高通量筛选和虚拟分子对接技术，以鉴定针对皮肤衰老中蛋白激酶C（PKC）的天然化合物。PKC抑制剂Go 6983对基质金属蛋白酶-1（MMP-1）转录表现出强效抑制作用。表没食子儿茶素没食子酸酯（EGCG）是显示出可显著降低HaCaT细胞中紫外线B（UVB）诱导的MMP-1表达的候选物之一，并且它对PKCα具有很强的亲和力。有趣的是，EGCG仅与PKCα结合，而不与δ和ζ亚型结合。阻断PKCα不会提高HaCaT细胞中UVB诱导的MMP-1表达。在人类皮肤模型中，EGCG阻止了由太阳光中的紫外线引起的胶原蛋白分解和表皮厚度变化。这表明EGCG在皮肤病学和药物开发中可能有用。这些发现突出了基于结构的筛选在鉴定可应用于化妆品、皮肤病学、预防保健和制药领域的候选化合物中的作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82b5/11567019/62689b9b47ea/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82b5/11567019/437d0a501673/ga1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82b5/11567019/da35d7825eb3/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82b5/11567019/56131239458f/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82b5/11567019/862c030fe75d/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82b5/11567019/13240dfb3bba/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82b5/11567019/62689b9b47ea/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82b5/11567019/437d0a501673/ga1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82b5/11567019/da35d7825eb3/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82b5/11567019/56131239458f/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82b5/11567019/862c030fe75d/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82b5/11567019/13240dfb3bba/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82b5/11567019/62689b9b47ea/gr5.jpg

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Food Sci Biotechnol. 2023 Jul 3;33(4):955-967. doi: 10.1007/s10068-023-01362-6. eCollection 2024 Mar.
2
Cutaneous Components Leading to Pruritus, Pain, and Neurosensitivity in Atopic Dermatitis: A Narrative Review.特应性皮炎中导致瘙痒、疼痛和神经敏感性的皮肤成分：一项叙述性综述
Dermatol Ther (Heidelb). 2024 Jan;14(1):45-57. doi: 10.1007/s13555-023-01081-0. Epub 2024 Jan 6.
3
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Cell Signal. 2024 Jan;113:110966. doi: 10.1016/j.cellsig.2023.110966. Epub 2023 Nov 8.
4
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5
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