Bao Ming-Yang, Wang Zhe, Nuez-Ortín Waldo G, Zhao Guiping, Dehasque Marleen, Du Zhen-Yu, Zhang Mei-Ling
School of Life Sciences, East China Normal University, Shanghai 200241, China.
Adisseo SAS, 10 Pl, du Général de Gaulle, Antony 92160, France.
Aquac Nutr. 2024 Feb 7;2024:1518809. doi: 10.1155/2024/1518809. eCollection 2024.
Lysophospholipids (LPLs) and bile acids (BA) are commonly used as emulsifiers in aquaculture. This study investigated the effects of dietary supplementation of LPLs or BA on the growth performance, lipid deposition, and intestinal health of largemouth juveniles. Fish were randomly allotted into three groups in quadruplicate and fed with a basal diet (CON) or diets containing 300 mg/kg LPLs (LPLs), or 300 mg/kg commercially available BA product (BA) for 8 weeks. The results showed that compared with the control group, LPLs and BA supplemented groups showed a higher weight gain trend, and LPLs supplementation promoted the protein deposition in fish body. Both BA and LPLs supplementations helped to maintain liver health by decreasing the activities of aspartate aminotransferase and alanine aminotransferase in serum. Besides, LPLs supplementation decreased overall lipid deposition in terms of mesenteric fat index and liver lipid content. Furthermore, LPLs supplementation showed unique advantage in improving intestinal barrier, as characterized by the increased villus length and higher expression of the tight junction protein expression. LPLs supplementation also increased the alpha diversity index and the abundances of Proteobacteria in the intestinal microbiota which is positively correlated with the abundance of SCFA in the gut. These findings will promote the application of novel feed additives and especially provide a basis for the rational selection of emulsifiers in the aquaculture industry.
溶血磷脂（LPLs）和胆汁酸（BA）常用于水产养殖中的乳化剂。本研究调查了日粮中添加LPLs或BA对大口幼鱼生长性能、脂质沉积和肠道健康的影响。将鱼随机分为三组，每组四个重复，分别饲喂基础日粮（CON）或含有300 mg/kg LPLs的日粮（LPLs），或300 mg/kg市售BA产品的日粮（BA），持续8周。结果表明，与对照组相比，添加LPLs和BA的组体重增加趋势更高，添加LPLs促进了鱼体蛋白质沉积。添加BA和LPLs均有助于通过降低血清中天冬氨酸转氨酶和丙氨酸转氨酶的活性来维持肝脏健康。此外，就肠系膜脂肪指数和肝脏脂质含量而言，添加LPLs降低了总体脂质沉积。此外，添加LPLs在改善肠道屏障方面显示出独特优势，其特征是绒毛长度增加和紧密连接蛋白表达上调。添加LPLs还增加了肠道微生物群中的α多样性指数和变形菌门的丰度，这与肠道中短链脂肪酸的丰度呈正相关。这些发现将促进新型饲料添加剂的应用，特别是为水产养殖业中乳化剂的合理选择提供依据。
