Sauro Salvatore, Osorio Raquel, Watson Timothy F, Toledano Manuel
Dental Biomaterials and Minimally Invasive Dentistry, Departamento de Odontología, Facultad de Ciencias de la Salud, Universidad CEU-Cardenal Herrera, Valencia, Spain.
Dental Materials, School of Dentistry, University of Granada, Colegio Máximo, Campus de Cartuja, Granada, Spain.
Dent Mater. 2015 Jul;31(7):759-77. doi: 10.1016/j.dental.2015.03.013. Epub 2015 Apr 23.
The study aimed at evaluating the remineralization of acid-etched dentin pre-treated with primers containing biomimetic analogs and bonded using an ion-releasing light-curable resin-based material.
An experimental etch-and-rinse adhesive system filled with Ca(2+), PO4(3-)-releasing Ca-Silicate micro-fillers was created along with two experimental primers containing biomimetic analogs such as sodium trimetaphosphate (TMP) and/or polyaspartic acid (PLA). Dentin specimens etched with 37% H3PO4 were pre-treated with two different aqueous primers containing the polyanionic biomimetic analogs or deionized water and subsequently bonded using the experimental resin-based materials. The specimens were sectioned and analyzed by AFM/nanoindentation to evaluate changes in the modulus of elasticity (Ei) across the resin-dentin interface at different AS storage periods (up to 90 days). Raman cluster analysis was also performed to evaluate the chemical changes along the interface. The phosphate uptake by the acid-etched dentin was evaluated using the ATR-FTIR. Additional resin-dentin specimens were tested for microtensile bond strength. SEM examination was performed after de-bonding, while confocal laser microscopy was used to evaluate the interfaces ultramorphology and micropermeability.
Both biomimetic primers induced phosphate uptake by acid-etched dentin. Specimens created with the ion-releasing resin in combination with the pre-treatment primers containing either PLA and TMA showed the greatest recovery of the Ei of the hybrid layer, with no decrease in μTBS (p>0.05) after 3-month AS storage. The ion-releasing resin applied after use of the biomimetic primers showed the greatest reduction in micropermeability due to mineral precipitation; these results were confirmed using SEM.
The use of the ion-releasing resin-based system applied to acid-etched dentin pre-treated with biomimetic primers containing analogs of phosphoproteins such as poly-l-aspartic acid and/or sodium trimetaphosphate provides a suitable bonding approach for biomimetic remineralization of resin-dentin interfaces.
本研究旨在评估用含有仿生类似物的底漆预处理并用离子释放型光固化树脂基材料粘结的酸蚀牙本质的再矿化情况。
创建了一种填充有释放Ca(2+)、PO4(3-)的硅酸钙微填料的实验性酸蚀冲洗粘结系统，以及两种含有仿生类似物（如三聚偏磷酸钠（TMP）和/或聚天冬氨酸（PLA））的实验性底漆。用37% H3PO4蚀刻的牙本质标本先用两种不同的含有聚阴离子仿生类似物的水性底漆或去离子水进行预处理，随后用实验性树脂基材料粘结。将标本切片并通过原子力显微镜/纳米压痕分析，以评估在不同人工唾液（AS）储存期（长达90天）内树脂-牙本质界面处弹性模量（Ei）的变化。还进行了拉曼聚类分析，以评估沿界面的化学变化。使用衰减全反射傅里叶变换红外光谱（ATR-FTIR）评估酸蚀牙本质对磷酸盐的摄取。对额外的树脂-牙本质标本进行微拉伸粘结强度测试。脱粘后进行扫描电子显微镜（SEM）检查，同时使用共聚焦激光显微镜评估界面的超微形态和微渗透性。
两种仿生底漆均诱导酸蚀牙本质摄取磷酸盐。用离子释放树脂与含有PLA和TMA的预处理底漆组合制备的标本显示，混合层的Ei恢复最大，在3个月AS储存后微拉伸粘结强度（μTBS）没有降低（p>0.05）。使用仿生底漆后应用的离子释放树脂由于矿物质沉淀而显示出微渗透性的最大降低；这些结果通过SEM得到证实。
将离子释放树脂基系统应用于用含有聚-L-天冬氨酸和/或三聚偏磷酸钠等磷蛋白类似物仿生底漆预处理的酸蚀牙本质，为树脂-牙本质界面的仿生再矿化提供了一种合适的粘结方法。
