Deg J K, Binder P S
Curr Eye Res. 1986 Jan;5(1):27-36. doi: 10.3109/02713688608995162.
Since scanning electron microscopy (SEM) is commonly used to evaluate hydrophilic lens deposits, it is important to know what artifacts are induced by the various techniques used to process the lenses. We performed scanning electron microscopy (SEM) using never-worn lenses with hydrations from 38.6% to 71%. Each lens was taken from its vial of factory-sealed, sterile-preserved saline and processed for SEM examination using liquid CO2, freon critical point drying, or air drying with or without alcohol dehydration. The lenses revealed abnormal surface structures occasionally resembling deposits reported on worn soft contact lenses. Varying degrees of gas pockets and surface wrinkling were seen. Individual polymers and lens hydration reacted differently to the various techniques of processing. Of the four methods evaluated, air drying without alcohol dehydration induced the least artifacts.
由于扫描电子显微镜（SEM）通常用于评估亲水性镜片沉积物，因此了解用于处理镜片的各种技术会产生哪些伪像非常重要。我们使用含水量从38.6%到71%的全新镜片进行了扫描电子显微镜（SEM）检查。每个镜片都从其工厂密封、无菌保存的生理盐水小瓶中取出，并使用液态二氧化碳、氟利昂临界点干燥或有无酒精脱水的空气干燥进行处理，以用于SEM检查。这些镜片显示出异常的表面结构，偶尔类似于佩戴过的软性隐形眼镜上报告的沉积物。观察到不同程度的气穴和表面起皱。不同的聚合物和镜片含水量对各种处理技术的反应不同。在评估的四种方法中，无酒精脱水的空气干燥产生的伪像最少。
