Bee cups 2.0: P-cups as single-use cages for honey bee (Hymenoptera: Apidae) experiments.

Honey bees and other pollinators face threats from pesticides, imperfect nutrition, and a diverse set of parasites and pathogens. Honey bees are also a research model for development, social behavior, microbiology, and aging. Tackling these questions requires a mix of in-hive and controlled laboratory experiments. We have perfected small-scale, inexpensive, disposable, and rearing arenas for honey bees that have proved useful for hundreds of bioassays with thousands of bees. We describe those arenas here, show their advantages over current hoarding cages, and provide videos demonstrating their many uses.