研究星鲽的性别决定揭示了鲽形目鱼类的性染色体进化，并鉴定出一个性别特异性标记。

Investigation of sex determination in starry flounder ( ) reveals sex chromosome evolution in Pleuronectiformes and identifies a sex-specific marker.

机构信息

State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong 266071, China.

School of Graduate, Chinese Academy of Agricultural Sciences, Beijing 100081, China.

出版信息

Zool Res. 2024 Nov 18;45(6):1347-1356. doi: 10.24272/j.issn.2095-8137.2024.078.

DOI:10.24272/j.issn.2095-8137.2024.078

PMID:39572017

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11668952/

Abstract

The identification of sex chromosomes is fundamental for exploring the mechanism and evolution of sex determination. , a species exhibiting clear sexual dimorphism and homomorphic chromosome pairs, has received limited research concerning its sex determination mechanisms. Clarifying the sex chromosome of will enhance our understanding of sex chromosome evolution in Pleuronectiformes. This study employed whole-genome resequencing to investigate the sex chromosome and sex determination system in . Notably, Chr23 was identified as the sex chromosome in , with the sex-determining region (SDR) occupying 48.1% of the chromosome and featuring an XX/XY system. Sex chromosome turnover was observed within Pleuronectiformes, with , , and sharing a common ancestral karyotype. No inversions were detected within the SDR of , although chromosomal rearrangements between sex chromosomes and autosomes were identified. Additionally, a sex-specific marker for was ascertained, enabling genetic sex identification, with significant implications for improving breeding programs and aquaculture practices.

摘要

性染色体的鉴定对于探索性别决定的机制和进化至关重要。牙鲆是一种表现出明显性别二态性和同形染色体对的物种，但其性别决定机制的研究相对较少。阐明牙鲆的性染色体将有助于我们理解鲽形目动物性染色体的进化。本研究采用全基因组重测序技术，对牙鲆的性染色体和性别决定系统进行了研究。值得注意的是，Chr23 被鉴定为牙鲆的性染色体，性决定区（SDR）占据了染色体的 48.1%，呈现出 XX/XY 系统。在鲽形目动物中观察到了性染色体的翻转，牙鲆、大菱鲆和半滑舌鳎共享一个共同的祖先核型。在牙鲆的 SDR 中没有发现倒位，但在性染色体和常染色体之间发现了染色体重排。此外，还确定了牙鲆的一个性别特异性标记，实现了遗传性别鉴定，这对改进繁殖计划和水产养殖实践具有重要意义。