Resolving the Loss of Intermediate-Size Speech Aerosols in Funnel-Guided Particle Counting Measurement.

Modeling of airborne virus transmission and protection against it requires knowledge of the amount of biofluid emitted into the atmosphere and its viral load. Whereas viral concentrations in biofluids are readily measured by quantitative PCR, the total volume of fluids aerosolized during speaking, as measured by different researchers using different technologies, differs by several orders of magnitude. We compared collection methods in which the aerosols first enter into a low humidity chamber either by direct injection or via commonly used funnel and tubing arrangements, followed by standard optical particle sizer measurement. This "collect first, measure later" approach sacrifices recording of the temporal correlation between aerosol generation and sound types such as plosives and vowels. However, the direct-injection mode prevents inertia deposition associated with the funnel arrangements and reveals far more intermediate size (5-20 μm diameter) particles that can dominate the total mass of the ejected respiratory aerosol. The larger aerosol mass observed with our method partially reconciles the large discrepancy between the SARS-CoV-2 infectious dose estimated from superspreader event analyses and that from human challenge data. Our results also impact measures to combat airborne virus transmission because they indicate that aerosols that settle faster than good room ventilation rates can dominate this process.