Vallance S
Hum Nutr Clin Nutr. 1986 Jan;40(1):35-41.
The changes in buffy layer (BL) vitamin C concentration following surgical operations were studied in relation to changes in the leucocyte and platelet counts. It was found that the postoperative fall and subsequent changes in BL vitamin C concentration corresponded to changes in the platelet/leucocyte ratio (PLR) in whole blood and consequently in the buffy layer preparations from which vitamin C concentrations were measured. The results of the study showed that the fall in BL vitamin C measured following operations is an artifact of the method used rather than a demand for vitamin C. It highlights the importance of measuring the PLR, and suggests the need for a differential leucocyte count, when undertaking BL vitamin C analysis.
研究了外科手术后血沉棕黄层（BL）维生素C浓度的变化与白细胞和血小板计数变化的关系。发现术后BL维生素C浓度的下降及随后的变化与全血中血小板/白细胞比值（PLR）的变化相对应，因此也与测量维生素C浓度的血沉棕黄层制剂中的变化相对应。研究结果表明，手术后测量的BL维生素C下降是所用方法造成的假象，而非对维生素C的需求。这突出了测量PLR的重要性，并表明在进行BL维生素C分析时需要进行白细胞分类计数。
