Evans R M, Currie L, Campbell A
Ann Clin Biochem. 1980 Sep;17(5):252-5. doi: 10.1177/000456328001700507.
The leucocyte ascorbic acid content is widely used as a measure of tissue ascorbic acid status. Standard methods of analysis, however, isolate both leucocytes and platelets (buffy layer), with consequent overestimation, since platelet ascorbic acid is attributed to the leucocytes. Fourteen healthy individuals on ascorbic acid supplements and 11 patients on mega dose ascorbic acid therapy were studied. A significant correlation was demonstrated between the 'leucocyte' ascorbic acid content and the platelet: leucocyte ratio (r = 0.70, P < 0.001). It is suggested that changes in the relative distribution of platelets and leucocytes in the blood will result in an apparent change in the 'leucocyte' ascorbic acid content regardless of any actual change in the ascorbic acid content of the cells.
白细胞中的抗坏血酸含量被广泛用作衡量组织抗坏血酸状态的指标。然而，标准分析方法会同时分离白细胞和血小板（血沉棕黄层），从而导致高估结果，因为血小板中的抗坏血酸被归为白细胞的。对14名服用抗坏血酸补充剂的健康个体和11名接受大剂量抗坏血酸治疗的患者进行了研究。结果表明，“白细胞”抗坏血酸含量与血小板：白细胞比值之间存在显著相关性（r = 0.70，P < 0.001）。研究表明，血液中血小板和白细胞相对分布的变化将导致“白细胞”抗坏血酸含量出现明显变化，而无论细胞中抗坏血酸含量是否有任何实际变化。
