Schorah C J, Habibzadeh N, Hancock M, King R F
Ann Clin Biochem. 1986 Sep;23 ( Pt 5):566-70. doi: 10.1177/000456328602300513.
Plasma and buffy layer vitamin C concentrations have been measured in 19 patients before and following major surgery, and falls of 36% and 43% respectively were recorded on the first post-operative day. However, the change in the buffy layer could not be accounted for by a change in the concentration of vitamin C in platelets, mononuclear cells or polymorphonuclear leucocytes, the cell types which form the buffy layer. The buffy layer change could be explained by a post-surgical increase in the number of polymorphonuclear leucocytes which, when the buffy layer vitamin C is expressed per 10(8) white cells, dilutes the platelet and mononuclear cell contribution to the buffy layer vitamin C concentration. The fall in buffy layer vitamin C does not, therefore, reflect any increased demand for white cell vitamin C in the immediate post-operative period, although the decrease in plasma vitamin C could imply some increased utilisation or redistribution of the non-cellular compartment.
对19名患者在大手术前后测量了血浆和血沉棕黄层中的维生素C浓度，术后第一天分别记录到下降了36%和43%。然而，血沉棕黄层的变化不能用构成血沉棕黄层的细胞类型（血小板、单核细胞或多形核白细胞）中维生素C浓度的变化来解释。血沉棕黄层的变化可以通过手术后多形核白细胞数量的增加来解释，当按每10⁸个白细胞计算血沉棕黄层维生素C时，会稀释血小板和单核细胞对血沉棕黄层维生素C浓度的贡献。因此，血沉棕黄层维生素C的下降并不反映术后即刻白细胞对维生素C的需求增加，尽管血浆维生素C的下降可能意味着非细胞部分的利用或重新分布有所增加。
