Bamboo Research Institute, School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, China.
Sichuan Provincial Forestry and Grass Land Key Laboratory for Conservation and Sustainable Utilization of Bamboo Genetic Resources in Southwest of China, Mianyang, China.
BMC Plant Biol. 2024 Nov 22;24(1):1112. doi: 10.1186/s12870-024-05825-8.
Bamboo, as a timber plant, holds significant environmental and economic value. Dendrocalamus farinosus is particularly valuable as it serves both as a source of bamboo shoots and timber, offering high yield, strong disease resistance, and superior fiber quality. Our previous study demonstrated that bio-organic fertilizers promoted the growth of D. farinosus and significantly altered the cellulose and lignin content, key components of the secondary cell wall in culms. However, the underlying regulatory mechanisms remain unclear. In this study, we used metabolomic and transcriptomic analyses to uncover the potential mechanisms by which bio-organic fertilizers affect the secondary cell wall biosynthesis in D. farinosus. A total of 1,437 metabolites were identified, with 20 differential metabolites significantly enriched in the phenylpropanoid metabolic pathway in bamboo shoots (7 upregulated; 13 downregulated). We identified 8,075 differentially expressed genes in bamboo shoots, including 72 genes potentially involved in lignin and flavonoid biosynthesis (6 upregulated; 66 downregulated). In internodes, we identified 5,324 differentially expressed genes, including 83 genes potentially involved in secondary cell wall biosynthesis (43 upregulated; 39 downregulated). Quantitative real-time PCR (qRT-PCR) validated the expression patterns of 8 key genes in internodes. The results suggest that bio-organic fertilizers may affect secondary cell wall biosynthesis in internodes by inhibiting the phenylpropanoid metabolic pathway in D. farinosus shoots. Our study offers insights into the efficient utilization of bamboo and lignocellulosic biomass, serving as a valuable resource for future research.
竹子作为一种木材植物，具有重要的环境和经济价值。巨龙竹因其既是笋用竹又是材用竹，具有产量高、抗病性强、纤维品质优的特点而尤为珍贵。我们之前的研究表明，生物有机肥能促进巨龙竹的生长，显著改变竹秆次生细胞壁的纤维素和木质素含量，这是次生细胞壁的关键组成部分。然而，其潜在的调控机制尚不清楚。在这项研究中，我们使用代谢组学和转录组学分析来揭示生物有机肥影响巨龙竹次生细胞壁生物合成的潜在机制。共鉴定出 1437 种代谢物，其中 20 种差异代谢物在竹笋的苯丙烷代谢途径中显著富集（7 种上调；13 种下调）。我们在竹笋中鉴定出 8075 个差异表达基因，包括 72 个可能参与木质素和类黄酮生物合成的基因（6 个上调；66 个下调）。在节间中，我们鉴定出 5324 个差异表达基因，包括 83 个可能参与次生细胞壁生物合成的基因（43 个上调；39 个下调）。定量实时 PCR（qRT-PCR）验证了节间 8 个关键基因的表达模式。结果表明，生物有机肥可能通过抑制巨龙竹竹笋中的苯丙烷代谢途径来影响节间的次生细胞壁生物合成。本研究为高效利用竹子和木质纤维素生物质提供了新的见解，为未来的研究提供了有价值的资源。
