Osman S F, Fett W F, Fishman M L
J Bacteriol. 1986 Apr;166(1):66-71. doi: 10.1128/jb.166.1.66-71.1986.
Exopolysaccharides (EPS) of the soybean pathogen Pseudomonas syringae pv. glycinea were isolated from culture filtrates and infected soybean leaves. Levan (a polyfructan with a C-2----C-6 backbone and C-2----C-1 branching) or acetylated alginate (a linear polyuronide of C-1----C-4-linked mannuronic and guluronic acids) was isolated from culture filtrates when bacterial strains were grown in a semisynthetic medium containing sucrose or glucose, respectively, as the primary carbon source. Acetylated alginate was the only EPS isolated from soybean [Glycine max (L.) Merr.] leaves inoculated with compatible (disease-inducing) strains of P. syringae pv. glycinea. The acetyl content of the P. syringae pv. glycinea alginates varied from 3 to 14%, and the amount of guluronic acid varied from less than 1 to 20%. The P. syringae pv. glycinea alginates from in vitro batch cultures were of lower molecular weight and polydispersity than those from in planta cultures, and both were of lower molecular weights than alginates produced by Pseudomonas aeruginosa.
大豆病原菌丁香假单胞菌大豆致病变种（Pseudomonas syringae pv. glycinea）的胞外多糖（EPS）是从培养滤液和受感染的大豆叶片中分离得到的。当细菌菌株分别在以蔗糖或葡萄糖作为主要碳源的半合成培养基中生长时，可从培养滤液中分离得到果聚糖（一种具有C-2→C-6主链和C-2→C-1分支的多聚果糖）或乙酰化藻酸盐（一种由C-1→C-4连接的甘露糖醛酸和古洛糖醛酸组成的线性聚uronide）。乙酰化藻酸盐是从接种了丁香假单胞菌大豆致病变种的兼容（致病诱导）菌株的大豆[Glycine max (L.) Merr.]叶片中分离得到的唯一EPS。丁香假单胞菌大豆致病变种藻酸盐的乙酰含量在3%至14%之间变化，古洛糖醛酸的含量在不到1%至20%之间变化。体外分批培养的丁香假单胞菌大豆致病变种藻酸盐的分子量和多分散性低于植物体内培养的藻酸盐，且两者的分子量均低于铜绿假单胞菌产生的藻酸盐。
