Chander Chikine Yashas, Kumar Yata Prashanth, Vankayala Bhavana, Veeraiyan Mounika, Thomas Delphia, Yadav Sarjeev Singh
Department of Conservative Dentistry and Endodontics, Government Dental College and Hospital, Hyderabad, Telangana, India.
Department of Dentistry, All India Institute of Medical Sciences, Raipur, Chattishgarh, India.
J Conserv Dent Endod. 2024 Oct;27(10):1037-1041. doi: 10.4103/JCDE.JCDE_490_24. Epub 2024 Oct 5.
In root canal disinfection, intracanal medicaments play a vital role. Typical intracanal medicament, calcium hydroxide (CH), has the lowest efficacy against the biofilm of Enterococcus faecalis. Nanoparticles and their combinations may increase antimicrobial activity, making them better than conventional CH.
The aim is to determine the efficacy of nanoparticle CH (NPCH) and silver nanoparticles (AgNP) against E. faecalis.
A total of 60 premolars were chosen. The samples were cleaned and decoronated. Cleaning and shaping were done. The apices were tightly sealed with modeling wax before being injected with 5 μl of E. faecalis (ATCC 29212) bacterial suspension, and then left to incubate. Samples were categorized into CH (G-I), NPCH (G-II), AgNP (G-III), and a combination of NPCH + AgNP (G-IV) according to the intracanal medication used. Eppendorf tubes were used to transfer dentin chips after they had been incubated for 7 days. The process of obtaining bacterial colony-forming units (CFUs) involves inoculating sediments onto blood agar.
The findings are statistically significant, number of CFUs seen with the Combination of NPCH + AgNP is lower than the other experimental groups, the group treated with CH had the largest number of CFUs.
The highest antibacterial effect was seen in combination of NPCH + AgNP.
在根管消毒中，根管内药物起着至关重要的作用。典型的根管内药物氢氧化钙（CH）对粪肠球菌生物膜的疗效最低。纳米颗粒及其组合可能会增强抗菌活性，使其优于传统的CH。
目的是确定纳米颗粒CH（NPCH）和银纳米颗粒（AgNP）对粪肠球菌的疗效。
共选取60颗前磨牙。对样本进行清洁和去冠处理。进行根管清理和预备。在根尖用模型蜡紧密密封后，注入5μl粪肠球菌（ATCC 29212）细菌悬液，然后进行培养。根据所用的根管内药物，将样本分为CH组（G-I）、NPCH组（G-II）、AgNP组（G-III）和NPCH + AgNP组合组（G-IV）。培养7天后，用微量离心管转移牙本质碎屑。获得细菌菌落形成单位（CFU）的过程包括将沉淀物接种到血琼脂上。
研究结果具有统计学意义，NPCH + AgNP组合组观察到的CFU数量低于其他实验组，CH治疗组的CFU数量最多。
NPCH + AgNP组合显示出最高的抗菌效果。
