Belhouala Khadidja, Korkmaz Cansu, Taş Küçükaydın Meltem, Küçükaydın Selçuk, Duru Mehmet Emin, Benarba Bachir
Laboratory Research on Biological Systems and Geomatics, Mustapha Stambouli University of Mascara, Mascara 29001, Algeria.
Department of Biology, Faculty of Science, Muğla Sıtkı Koçman University, 48000 Muğla, Turkey.
ACS Omega. 2024 Nov 5;9(46):45719-45732. doi: 10.1021/acsomega.3c10407. eCollection 2024 Nov 19.
Growing in prominence is the utilization of natural product-based therapies, especially edibles used in traditional medicine, as more people seek natural and holistic approaches to health and well-being. This research aimed to determine the phenolic compounds, and antioxidant, anti-inflammatory, and anticancer effects of aqueous and methanolic extracts from (L.) Ach., a common spice in Algeria. HPLC-DAD was used to establish the phenolic profile, whereas the antioxidant activity was determined by DPPH, FRAP, phosphomolybdate, and hydrogen peroxide (HO) assays. Human red blood cell (HRBC) stabilization, albumin denaturation, and proteinase inhibition procedures were performed to investigate the anti-inflammatory activities, and an MTT assay was used to demonstrate the cytotoxic effect against three human cancer cell lines (HT-29, PC-3, A549) and human nontumor (CCD18-Co) cells. Our results showed that the major phenolics detected were mostly phenylpropanoids with domination of rosmarinic acid (79.17 mg/g), caffeic acid (46.52 mg/g), -cinnamic acid (29.23 mg/g), and chlorogenic acid (23.68 mg/g). In addition, six flavonoids were identified (1.98-11.34 mg/g), namely, luteolin, myricetin, kaempferol, rutin, apigenin, and quercetin. Other compounds were relatively present in both extracts, gallic acid and 3-hydroxybenzoic acid (phenolic acids), except pyrocatechol (benzenediol), which was slightly detected in the aqueous extract (0.91 mg/g). Moreover, both extracts showed strong antioxidant activity, with a higher antioxidant potential, as shown by the methanolic extract. Likewise, both reduced HRBC hemolysis damage and moderately suppressed protein denaturation, which reflected their anti-inflammatory potential. Interestingly, the methanolic extract significantly reduced the growth of HT-29, PC-3, and A549 cells by 67.03, 75.56, and 62.96% respectively. No cytotoxic effects were observed in the nontumor cells. The methanolic extract had the lowest IC values of 100 ± 0.04, 146 ± 0.05, and 112 ± 0.06 μg/mL against HT-29, PC-3, and A549 cell lines, respectively. In conclusion, , especially its methanolic extract, could be considered as a promising source of antioxidant and anticancer molecules.
随着越来越多的人寻求天然和整体的健康与幸福方法，基于天然产物的疗法，尤其是传统医学中使用的可食用物，正日益受到关注。本研究旨在确定阿尔及利亚一种常见香料迷迭香叶（L.）Ach. 的水提取物和甲醇提取物中的酚类化合物、抗氧化、抗炎和抗癌作用。采用高效液相色谱 - 二极管阵列检测法（HPLC - DAD）建立酚类图谱，而抗氧化活性则通过二苯基苦味酰基自由基（DPPH）、铁离子还原抗氧化能力（FRAP）、磷钼酸盐和过氧化氢（HO）测定法来确定。通过人红细胞（HRBC）稳定化、白蛋白变性和蛋白酶抑制程序来研究抗炎活性，并使用MTT法证明对三种人类癌细胞系（HT - 29、PC - 3、A549）和人类非肿瘤（CCD18 - Co）细胞的细胞毒性作用。我们的结果表明，检测到的主要酚类物质大多为苯丙烷类化合物，其中迷迭香酸（79.17 mg/g）、咖啡酸（46.52 mg/g）、反式肉桂酸（29.23 mg/g）和绿原酸（23.68 mg/g）占主导地位。此外，还鉴定出六种黄酮类化合物（1.98 - 11.34 mg/g），即木犀草素、杨梅素、山奈酚、芦丁、芹菜素和槲皮素。两种提取物中还相对含有其他化合物，如没食子酸和3 - 羟基苯甲酸（酚酸），除了儿茶酚（苯二酚），其在水提取物中略有检出（0.91 mg/g）。此外，两种提取物均表现出较强的抗氧化活性，甲醇提取物显示出更高的抗氧化潜力。同样，两种提取物都减少了HRBC溶血损伤并适度抑制了蛋白质变性，这反映了它们的抗炎潜力。有趣的是，甲醇提取物分别显著降低了HT - 29、PC - 3和A549细胞的生长，降低幅度分别为67.03%、75.56%和62.96%。在非肿瘤细胞中未观察到细胞毒性作用。甲醇提取物对HT - 29、PC - 3和A549细胞系的最低半数抑制浓度（IC）值分别为100 ± 0.04、146 ± 0.05和112 ± 0.06 μg/mL。总之，迷迭香叶，尤其是其甲醇提取物，可被认为是抗氧化和抗癌分子的一个有前途的来源。
