Takahashi Masayoshi, Nakazawa Masahiro, Nishimoto Takahiro, Odajima Mitsuyuki, Shirai Yasuyuki, Sugawa Shigetoshi
New Industry Creation Hatchery Center, Tohoku University, 6-6-10 Aoba, Aramaki, Aoba-ku, Sendai, Miyagi 980-8579, Japan.
Nakazawa Dental Clinic, 1-13-1 Yachiyodai-minami, Yachiyo, Chiba 276-0033, Japan.
Langmuir. 2024 Dec 10;40(49):25950-25956. doi: 10.1021/acs.langmuir.4c03339. Epub 2024 Nov 25.
In the field of medical implants, enhancing the wettability of artificial surfaces is crucial for improving biocompatibility. This study investigates the potential of ozone nanobubble water, an aqueous solution known for its strong oxidizing and sterilizing properties, to modify the surface of titanium dental implants. By immersing the implants in ozone nanobubble water for ∼10 min, we observed a significant transformation of their surface characteristics. Implant surfaces that had become hydrophobic over time, likely due to organic contaminants, became superhydrophilic, exhibiting a contact angle near zero. Fresh implant material is initially hydrophilic but becomes hydrophobic within a few days of drying. In sharp contrast, the hydrophilicity induced by ozone nanobubble water treatment persisted for more than one month. This durability suggests not only the removal of organic matter through cleaning but also a substantial alteration in the surface properties of the implants. The generation of ozone nanobubble water involved releasing ozone microbubbles into an aqueous solution containing trace amounts of iron and manganese, resulting in spherical particles with an average diameter of ∼10 nm. These particles could be bulk nanobubbles, a stabilized gas body surrounded by a solid shell composed of iron hydroxide. Termed "nanoshells" in our previous study, these particles demonstrated exceptional dispersibility without the need for stabilizing agents such as surfactants or capping agents, attributed to their inherent high wettability. The sustained hydrophilicity of the implant surfaces might be attributed to the adherence of these hydrophilic nanoshells to the implant's surface. This study highlights the potential of ozone nanobubble water for long-term surface modification of dental implants, offering a promising avenue for enhancing implant biocompatibility.
在医用植入物领域，提高人工表面的润湿性对于改善生物相容性至关重要。本研究探讨了以强氧化和杀菌性能著称的臭氧纳米泡水溶液对牙科钛植入物表面进行改性的潜力。通过将植入物浸泡在臭氧纳米泡水中约10分钟，我们观察到其表面特性发生了显著变化。随着时间推移因有机污染物而变得疏水的植入物表面变得超亲水，接触角接近零。新鲜的植入物材料最初是亲水的，但在干燥几天后就会变成疏水的。与之形成鲜明对比的是，经臭氧纳米泡水处理诱导的亲水性持续了一个多月。这种耐久性不仅表明通过清洁去除了有机物，还意味着植入物表面性质发生了实质性改变。臭氧纳米泡水的生成过程是将臭氧微泡释放到含有微量铁和锰的水溶液中，从而产生平均直径约为10纳米的球形颗粒。这些颗粒可能是体相纳米泡，即由氢氧化铁构成的固体壳包围的稳定气体体。在我们之前的研究中称这些颗粒为“纳米壳”，它们表现出优异的分散性，无需表面活性剂或封端剂等稳定剂，这归因于其固有的高润湿性。植入物表面持续的亲水性可能归因于这些亲水纳米壳附着在植入物表面。本研究突出了臭氧纳米泡水在牙科植入物长期表面改性方面的潜力，为提高植入物生物相容性提供了一条有前景的途径。
