Horikawa Hidehiro, Yui Tomoo, Nakanishi Yasuhiro, Hirose Yukito, Kado Takashi, Nezu Takashi, Oh Hourei, Ochi Morio
Division of Fixed Prosthodontics and Oral Implantology, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido, Tobetsu 061-0293, Japan.
Division of Clinical Cariology and Endodontology, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido, Tobetsu 061-0293, Japan.
Materials (Basel). 2025 Jul 3;18(13):3156. doi: 10.3390/ma18133156.
The biological aging of titanium implants, marked by increased surface hydrophobicity and organic contamination, reduces bioactivity and delays osseointegration. A major challenge in implant dentistry is determining how to preserve surface hydrophilicity during storage, as conventional atmospheric conditions accelerate surface degradation. This pilot in vivo study aimed to evaluate ozone nanobubble water (NBW3) as a storage medium to prevent biological aging and enhance the early-stage osseointegration of glow discharge-treated titanium implants. Screw-type implants were stored in either NBW3 or atmospheric conditions and then implanted into femoral bone defects in Sprague Dawley rats. Removal torque testing, scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), and histological analysis of bone-to-implant contact (BIC) were performed 14 and 28 days post-implantation. At 14 days, the NBW3-stored implants demonstrated significantly higher removal torque (2.08 ± 0.12 vs. 1.37 ± 0.20 N·cm), BIC (65.74 ± 12.65% vs. 44.04 ± 14.25%), and Ca/P atomic ratio (1.20 ± 0.32 vs. 1.00 ± 0.22) than the controls. These differences were not observed at 28 days, indicating NBW3's primary role in accelerating early osseointegration. The findings suggest that using NBW3 is a simple, effective approach to maintain implant surface bioactivity during storage, potentially improving clinical outcomes under early or immediate loading protocols.
钛植入物的生物老化表现为表面疏水性增加和有机污染，会降低生物活性并延迟骨整合。种植牙科的一个主要挑战是确定如何在储存过程中保持表面亲水性，因为传统的大气条件会加速表面降解。这项体内初步研究旨在评估臭氧纳米气泡水（NBW3）作为一种储存介质，以防止生物老化并增强经辉光放电处理的钛植入物的早期骨整合。将螺钉型植入物储存在NBW3或大气条件下，然后植入Sprague Dawley大鼠的股骨骨缺损中。在植入后14天和28天进行移除扭矩测试、扫描电子显微镜（SEM）、能量色散X射线光谱（EDX）以及骨与植入物接触（BIC）的组织学分析。在14天时，与对照组相比，储存在NBW3中的植入物表现出显著更高的移除扭矩（2.08±0.12对1.37±0.20 N·cm）、BIC（65.74±12.65%对44.04±14.25%）和钙/磷原子比（1.20±0.32对1.00±0.22）。在28天时未观察到这些差异，表明NBW3在加速早期骨整合中的主要作用。研究结果表明，使用NBW3是在储存过程中维持植入物表面生物活性的一种简单、有效的方法，可能会改善早期或即刻加载方案下的临床结果。
