Department of Chemistry, Sardar Vallabhbhai National Institute of Technology, Surat, Gujarat, India.
Department of Chemistry, Research Institute of Chem-Bio Diagnostic Technology, Chung-Ang University, Seoul, Dongjak-gu, Republic of Korea.
Luminescence. 2024 Nov;39(11):e70040. doi: 10.1002/bio.70040.
In this work, a facile and rapid fluorescence turn-off-on sensing method was developed for the detection of Pb and metalaxyl fungicide in environmental samples using yellow carbon dots (CDs) from Cordia dichotoma fruits. The prepared C. dichotoma-CDs showed the emission band (𝜆) at 535 nm under 448 nm of excitation wavelength. The fluorescence intensity of C. dichotoma-CDs quenches by Pb ion and then restores by metalaxyl fungicide, favoring to develop a fluorescence turn-off-on sensing strategy. The intensity ratios (I/I and I/I) are linear against Pb (0.01-50 μM) and metalaxyl fungicide (0.025-10 μM) concentrations, offering the detection limits of 0.0054 and 0.0087 μM for Pb and metalaxyl fungicide, respectively. Additionally, the potential application of C. dichotoma-CD-based fluorescence turn-off-on sensing approach was effectively demonstrated for Pb and metalaxyl fungicide assays in real samples, which confirms that C. dichotoma-CDs act as "turn-off-on" fluorescent probe for assaying of Pb and metalaxyl fungicide, respectively.
在这项工作中，我们开发了一种简便、快速的荧光关闭-开启传感方法，用于使用来自黄槐果实的黄色碳点 (CD) 检测环境样品中的 Pb 和金属杀菌剂。所制备的 C. dichotoma-CDs 在 448nm 的激发波长下显示出 535nm 的发射带 (𝜆)。C. dichotoma-CDs 的荧光强度被 Pb 离子猝灭，然后被金属杀菌剂恢复，有利于开发荧光关闭-开启传感策略。强度比 (I/I 和 I/I) 与 Pb(0.01-50 μM) 和金属杀菌剂 (0.025-10 μM) 浓度呈线性关系，分别为 Pb 和金属杀菌剂提供了 0.0054 和 0.0087 μM 的检测限。此外，基于 C. dichotoma-CD 的荧光关闭-开启传感方法的潜在应用在实际样品中的 Pb 和金属杀菌剂测定中得到了有效证明，这证实了 C. dichotoma-CDs 分别作为测定 Pb 和金属杀菌剂的“关闭-开启”荧光探针。
