Gökçe Halise Betül, Aslan İsmail
Department of Pharmaceutical Technology, Faculty of Pharmacy, Afyonkarahisar Health Sciences University, Afyonkarahisar 03030, Turkey.
Department of Pharmaceutical Technology, Hamidiye Faculty of Pharmacy, University of Health Sciences, Istanbul 34668, Turkey.
Gels. 2024 Nov 15;10(11):746. doi: 10.3390/gels10110746.
In recent years, in addition to the positive effects of probiotics and prebiotics on health, increasing research has shown that postbiotics also have significant potential in the health field. Postbiotics are bioactive components produced by probiotic bacteria during fermentation and may exhibit antimicrobial activity. This study investigated the antimicrobial effects of liposomal postbiotics formulated in gel. Various postbiotic-containing liposomal systems have been developed and optimized to prepare formulations. Optimized liposomes and liposomal postbiotic-containing gel forms were examined in terms of particle size, polydispersity index, zeta potential, structural properties, encapsulation efficiency, permeability, release profiles, and stability. Finally, the antimicrobial activities of the postbiotics and the optimum gel formulation LG1 were evaluated on , , , , and strains using disk diffusion and microdilution methods. The optimum liposome formulation L1 was determined to have a particle size of 185.32 ± 0.80 nm, a polydispersity index of 0.206 ± 0.012, a zeta potential of 35.0 ± 0.5 mV, and an encapsulation efficiency of 17.52%. Its permeability was determined as 51.52% at the end of 6 h. In vitro release studies showed that the drug release profile was in accordance with first-order kinetics and suitable for controlled release. The findings show that formulated postbiotics have similar antimicrobial activity to free postbiotics. These results suggest that liposomal gel formulations support the antimicrobial effects of postbiotics while providing advantages of use. In conclusion, the findings contribute to a better understanding of the antimicrobial potential of postbiotics and lipogelosomal postbiotics and optimize their use in pharmaceutical applications.
近年来，除了益生菌和益生元对健康的积极影响外，越来越多的研究表明，后生元在健康领域也具有巨大潜力。后生元是益生菌在发酵过程中产生的生物活性成分，可能具有抗菌活性。本研究调查了凝胶剂型脂质体后生元的抗菌效果。已开发并优化了各种含后生元的脂质体系统以制备制剂。从粒径、多分散指数、zeta电位、结构性质、包封率、渗透率、释放曲线和稳定性等方面对优化后的脂质体和含脂质体后生元的凝胶剂型进行了研究。最后，采用纸片扩散法和微量稀释法，对后生元和最佳凝胶制剂LG1对金黄色葡萄球菌、大肠杆菌、枯草芽孢杆菌、白色念珠菌和黑曲霉的抗菌活性进行了评价。确定最佳脂质体制剂L1的粒径为185.32±0.80nm，多分散指数为0.206±0.012，zeta电位为35.0±0.5mV，包封率为17.52%。6小时结束时其渗透率为51.52%。体外释放研究表明，药物释放曲线符合一级动力学，适合控释。研究结果表明，配制后的后生元与游离后生元具有相似的抗菌活性。这些结果表明，脂质体凝胶制剂在支持后生元抗菌作用的同时，还具有使用优势。总之，这些发现有助于更好地理解后生元和脂质体后生元的抗菌潜力，并优化它们在药物应用中的使用。
