Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Research Institute of Stomatology, Nanjing University, Nanjing 210008, China.
Dept. of Stomatology, Nanjing Honghua Community Health Service Center, Nanjing 210008, China.
Hua Xi Kou Qiang Yi Xue Za Zhi. 2024 Dec 1;42(6):716-722. doi: 10.7518/hxkq.2024.2024150.
To explore and analyze the feasibility of using indocyanine green (ICG) near-infrared fluorescence (NIF) imaging technology for the early diagnosis of oral potential malignant disorders and oral squamous cell carcinoma.
7,12-Dimethylbenz[a]anthracene in acetone solution was used to induce various pathological models of buccal mucosal lesions (mild/moderate dysplasia, severe dysplasia, squamous cell carcinoma) in golden hamster. ICG-NIF was conducted for the quantitative analysis of the fluorescence signal of lesion tissue, and evaluation of the diagnostic and discriminative capabilities of the ICG-NIF technology for mucosal lesions in various pathological states. Immunohistochemical staining was perform to examine the microvessel density (MVD) and microlymphatic vessel density (MLVD) of mucosa in various pathological states and explore the histological reasons underlying the differences in fluorescence signals.
The results of ICG-NIF fluorescence quantitative analysis reveal the higher fluorescence intensity of mucosal lesions in the experimental group compared with that of the normal mucosa on the control side, with statistical differences (<0.05). Moreover, the more severe the malignancy of mucosal lesions in the experimental group, the higher the fluorescence intensity. According to histopathological analysis, the malignant progression of mucosal lesions in golden hamsters was accompanied with an increase in MVD (<0.05) and a decrease in MLVD (<0.05).
The abnormal proliferation of mucosal lesions in golden hamsters exhibits a difference in ICG-NIF fluorescence signal compared with normal mucosal tissue. Fluorescence quantitative analysis methods can provide assistance in differentiation and show potential for clinical applications.
探讨和分析近红外荧光（NIF）成像技术中吲哚菁绿（ICG）在口腔潜在恶性疾病和口腔鳞状细胞癌早期诊断中的应用价值。
采用丙酮溶液中的 7,12-二甲基苯并[a]蒽诱导金黄地鼠颊黏膜病变的各种病理模型（轻度/中度异型增生、重度异型增生、鳞状细胞癌），对病变组织的荧光信号进行 ICG-NIF 定量分析，并评价 ICG-NIF 技术对不同病理状态黏膜病变的诊断和鉴别能力。免疫组织化学染色检测不同病理状态下黏膜的微血管密度（MVD）和微淋巴管密度（MLVD），探讨荧光信号差异的组织学原因。
ICG-NIF 荧光定量分析结果显示，实验组黏膜病变的荧光强度明显高于对照组对侧正常黏膜，差异有统计学意义（<0.05）。而且，实验组黏膜病变的恶性程度越严重，荧光强度越高。根据组织病理学分析，金黄地鼠口腔黏膜病变的恶性进展伴随着 MVD 的增加（<0.05）和 MLVD 的减少（<0.05）。
金黄地鼠黏膜病变的异常增殖与正常黏膜组织的 ICG-NIF 荧光信号存在差异。荧光定量分析方法有助于鉴别，具有潜在的临床应用价值。
