Division of Anatomy, School of Medical Sciences, University of Phayao, 56000 Mueang Phayao, Thailand.
Electron Microscopy Unit, Department of Anatomy, Faculty of Medicine, Khon Kaen University, 40002 Mueang, Khon Kaen, Thailand.
Front Biosci (Landmark Ed). 2024 Nov 19;29(11):388. doi: 10.31083/j.fbl2911388.
The biological activities of sulfated polysaccharides (SP) are well-documented, especially regarding wound healing. Sulfated galactan (SG), a type of SP extracted from the red seaweed , has been identified as having multiple therapeutic properties related to its wound healing capacity. Recent research indicates that degraded SG (DSG) from , when combined with octanoyl ester (DSGO), can improve wound healing in fibroblasts. However, the effectiveness of natural products in clinical settings often differs from results. This study aimed to develop and evaluate ointments containing DSG and DSGO for skin repair in an animal model.
Twenty-four Wistar rats were divided into four groups: (1) normal control, (2) ointment control, (3) DSG ointment, and (4) DSGO ointment. After inducing full-thickness excision wounds, these ointments were applied to the wounds. Wound contraction rate, histopathology, and protein related wound healing expression were then elucidated.
Our findings showed that both DSG and DSGO ointments significantly enhanced wound closure compared to the control groups. Histopathological and biochemical analyses indicated increased extracellular matrix production and fibroblasts, marked by improved fibroblast activity, neovascularization, and collagen deposition. Furthermore, immunohistochemistry and immunoblot analysis revealed that the ointments altered the expression of Ki67, α-smooth muscle actin (α-SMA), E-cadherin, vimentin, collagen, and components of the Smad signaling pathway, all of which are crucial for wound healing. The results also suggested that the DSGO ointment was marginally more effective in promoting wound healing in this model.
These results indicate that ointment supplemented with DSG and DSGO have the potential to enhance skin repair by improving histopathology and altering wound healing-related proteins.
硫酸化多糖（SP）的生物活性已有充分的文献记载，尤其是在伤口愈合方面。硫酸半乳聚糖（SG）是从红海藻中提取的一种 SP，已被确定具有多种与伤口愈合能力相关的治疗特性。最近的研究表明，来自 的降解硫酸半乳聚糖（DSG）与辛酰酯（DSGO）结合后，可改善成纤维细胞的伤口愈合。然而，天然产物在临床环境中的有效性往往与实验室结果不同。本研究旨在开发和评估含有 DSG 和 DSGO 的软膏，以在动物模型中促进皮肤修复。
将 24 只 Wistar 大鼠分为四组：（1）正常对照组，（2）软膏对照组，（3）DSG 软膏组，（4）DSGO 软膏组。在诱导全层切除伤口后，将这些软膏应用于伤口。然后阐明伤口收缩率、组织病理学和与蛋白质相关的伤口愈合表达。
我们的研究结果表明，与对照组相比，DSG 和 DSGO 软膏均显著促进了伤口闭合。组织病理学和生化分析表明，细胞外基质产生和成纤维细胞增加，表现为成纤维细胞活性、新生血管形成和胶原蛋白沉积得到改善。此外，免疫组化和免疫印迹分析显示，软膏改变了 Ki67、α-平滑肌肌动蛋白（α-SMA）、E-钙黏蛋白、波形蛋白、胶原蛋白和 Smad 信号通路成分的表达，这些都是伤口愈合的关键因素。结果还表明，在该模型中，DSGO 软膏在促进伤口愈合方面的效果略优。
这些结果表明，补充 DSG 和 DSGO 的软膏通过改善组织病理学和改变与伤口愈合相关的蛋白质，有可能增强皮肤修复。
