Giannini S H, Schittini M, Keithly J S, Warburton P W, Cantor C R, Van der Ploeg L H
Science. 1986 May 9;232(4751):762-5. doi: 10.1126/science.3961502.
Chromosomes of four species of Leishmania represented by ten different geographic isolates were analyzed by pulsed field gradient gel electrophoresis (PFG) to assess chromosome stability in these parasitic protozoans. Among different geographic isolates of the same subspecies, more than two-thirds of chromosomes had similar sizes, ethidium bromide staining intensities, and locations of alpha,beta-tubulin genes. However, among New World Leishmania, members of different species or subspecies have fewer than one-third of their chromosomes in common. Therefore, PFG karyotypes of Leishmania exhibit intraspecific variability similar to that reported for other parasitic protozoans. The greater similarities of the karyotypes of members of the same Leishmania subspecies may indicate that they represent valid taxa. These similarities also allowed the use of PFG in clinical diagnosis for rapid and accurate typing of patient isolates.
通过脉冲场梯度凝胶电泳（PFG）分析了由十种不同地理分离株代表的四种利什曼原虫的染色体，以评估这些寄生原生动物的染色体稳定性。在同一亚种的不同地理分离株中，超过三分之二的染色体具有相似的大小、溴化乙锭染色强度以及α、β-微管蛋白基因的位置。然而，在新大陆利什曼原虫中，不同物种或亚种的成员共有不到三分之一的染色体。因此，利什曼原虫的PFG核型表现出种内变异性，类似于其他寄生原生动物的报道。同一利什曼原虫亚种成员的核型具有更大的相似性，这可能表明它们代表有效的分类单元。这些相似性也使得PFG可用于临床诊断，以便对患者分离株进行快速准确的分型。
