Hu Shenqiang, Li Xiaopeng, Qing Enhua, Wang Junqi, Chen Qingliang, Song Yang, Chen Jiasen, Hu Jiwei, Li Liang, Wang Jiwen
State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, PR China; Key Laboratory of Livestock and Poultry Multi-Omics Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, PR China; Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, PR China; Key Laboratory of Agricultural Bioinformatics, Ministry of Education, Sichuan Agricultural University, Chengdu, Sichuan, PR China.
State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, PR China; Key Laboratory of Livestock and Poultry Multi-Omics Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, PR China; Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, PR China; Key Laboratory of Agricultural Bioinformatics, Ministry of Education, Sichuan Agricultural University, Chengdu, Sichuan, PR China.
Poult Sci. 2025 Jan;104(1):104589. doi: 10.1016/j.psj.2024.104589. Epub 2024 Nov 25.
As an important non-genetic factor, the rearing system has significant effects on male poultry reproductive system development. However, compared with other poultry such as chickens and ducks, less is known about the effects and mechanisms of rearing system on the gander reproductive organ development and semen quality. In the present study, the testicular morphological, histological, and transcriptomic responses of three goose breeds to the two dryland rearing systems (i.e., cage rearing system, CRS and net-floor mixed rearing system, MRS) were systematically analyzed and compared. Results from histomorphological analysis demonstrated that the effects of rearing system on the gander testicular development were age-dependent, and moreover, the CRS may be more conducive than MRS to the testicular development and semen quality during the period from post-hatch week 10 to week 43. At week 30, compared to Sichuan White goose (SW), the rearing system showed more pronounced effects on the testicular size, weight, and organ index of Gang goose (GE) and Landes goose (LD). However, such effects were mitigated in LD and even reversed in GE at week 43. Meanwhile, most testicular histological parameters of three goose breeds were higher under MRS than under CRS at week 30, while the converse was seen in some histological parameters of either GE or LD at week 43. Moreover, the semen quality was generally better under CRS than under MRS at week 43. Through comparative transcriptomics analysis, the Wnt signaling pathway together with several involved hub genes were identified to have important roles in mediating the effects of rearing system on the goose testicular development. Moreover, the metabolism-related, cell cycle, and Wnt signaling pathways could be partially responsible for differences in the goose breed-related testicular development and semen quality under CRS, where a number of genes involved in meiosis could have crucial roles. These results would not only provide novel insights into the effects and mechanisms of rearing system on male poultry reproductive performance, but they would also be helpful for the optimization and selection of dryland rearing systems in male geese.
作为一个重要的非遗传因素，饲养系统对雄性家禽生殖系统的发育有显著影响。然而，与鸡和鸭等其他家禽相比，关于饲养系统对雄鹅生殖器官发育和精液质量的影响及机制，我们了解得较少。在本研究中，系统地分析和比较了三个鹅品种对两种旱地饲养系统（即笼养系统，CRS和网上平养系统，MRS）的睾丸形态、组织学和转录组反应。组织形态学分析结果表明，饲养系统对雄鹅睾丸发育的影响具有年龄依赖性，此外，在孵化后第10周 至第43周期间，CRS可能比MRS更有利于睾丸发育和精液质量。在第30周时，与四川白鹅（SW）相比，饲养系统对雁鹅（GE）和朗德鹅（LD）的睾丸大小、重量和器官指数的影响更为明显。然而，在第43周时，这种影响在LD中减弱，在GE中甚至逆转。同时，在第30周时，三个鹅品种的大多数睾丸组织学参数在MRS下高于CRS，而在第43周时，GE或LD的一些组织学参数则相反。此外，在第43周时，CRS下的精液质量总体上优于MRS。通过比较转录组学分析，发现Wnt信号通路以及几个相关的枢纽基因在介导饲养系统对鹅睾丸发育的影响中具有重要作用。此外，代谢相关、细胞周期和Wnt信号通路可能部分解释了CRS下与鹅品种相关的睾丸发育和精液质量差异，其中一些参与减数分裂的基因可能起关键作用。这些结果不仅将为饲养系统对雄性家禽繁殖性能的影响及机制提供新的见解，也将有助于优化和选择雄鹅的旱地饲养系统。
