Ren Shuning, Xie Shijie, Peng Chen
College of Veterinary Medicine (CVM), China Agricultural University, Beijing, China.
Methods Mol Biol. 2025;2860:341-351. doi: 10.1007/978-1-0716-4160-6_21.
Lumpy skin disease virus (LSDV), a member of the capripoxvirus genus, induces lumpy skin disease (LSD) in cattle and buffalo populations. The initial documentation of LSD dates back to 1929 in Zambia, with subsequent rapid dissemination within the cattle community in Africa and Asia. Described as the "smallpox" equivalent in cattle, LSD manifests through clinical features such as fever, extensive cutaneous nodules, body wasting, lymph node enlargement, and skin edema. Isolation of LSDV constitutes an important step in evaluating virulence and implementing disease control measures. Skin samples obtained from afflicted cattle serve as the primary source for LSDV isolation due to its tropism for epidermal cells. Other tissues, including blood, lung, spleen, and saliva samples, are also employed for viral isolation. This chapter presents protocols for the convenient isolation of LSDV, relying on the plaque formation phenotype and utilizing MDBK cells. Emphasis is placed on stringent quality control methodologies to identify isolated viruses through multiple techniques, facilitating further investigations such as the in vitro growth of isolated LSDV.
结节性皮肤病病毒（LSDV）是山羊痘病毒属的成员，可在牛和水牛群体中引发结节性皮肤病（LSD）。LSD的首次记录可追溯到1929年的赞比亚，随后在非洲和亚洲的牛群中迅速传播。LSD被称为牛的“天花”，其临床表现为发热、广泛的皮肤结节、身体消瘦、淋巴结肿大和皮肤水肿。LSDV的分离是评估病毒毒力和实施疾病控制措施的重要步骤。由于LSDV对表皮细胞具有嗜性，从患病牛身上获取的皮肤样本是LSDV分离的主要来源。其他组织，包括血液、肺、脾脏和唾液样本，也用于病毒分离。本章介绍了依靠噬斑形成表型并利用MDBK细胞方便地分离LSDV的方案。重点是严格的质量控制方法，通过多种技术鉴定分离出的病毒，以便于进行进一步的研究，如分离出的LSDV的体外生长研究。
