Reddy Panga J, Sun Zhi, Wippel Helisa H, Baxter David H, Swearingen Kristian, Shteynberg David D, Midha Mukul K, Caimano Melissa J, Strle Klemen, Choi Yongwook, Chan Agnes P, Schork Nicholas J, Varela-Stokes Andrea S, Moritz Robert L
Institute for Systems Biology, Seattle, Washington, USA.
Department of Medicine, UConn Health, Farmington, Connecticut, USA.
Sci Data. 2024 Dec 2;11(1):1313. doi: 10.1038/s41597-024-04047-9.
Lyme disease is caused by an infection with the spirochete Borrelia burgdorferi, and is the most common vector-borne disease in North America. B. burgdorferi isolates harbor extensive genomic and proteomic variability and further comparison of isolates is key to understanding the infectivity of the spirochetes and biological impacts of identified sequence variants. Here, we applied both transcriptome analysis and mass spectrometry-based proteomics to assemble peptide datasets of B. burgdorferi laboratory isolates B31, MM1, and the infective isolate B31-5A4, to provide a publicly available Borrelia PeptideAtlas. Included are total proteome, secretome, and membrane proteome identifications of the individual isolates. Proteomic data collected from 35 different experiment datasets, totaling 386 mass spectrometry runs, have identified 81,967 distinct peptides, which map to 1,113 proteins. The Borrelia PeptideAtlas covers 86% of the total B31 proteome of 1,291 protein sequences. The Borrelia PeptideAtlas is an extensible comprehensive peptide repository with proteomic information from B. burgdorferi isolates useful for Lyme disease research.
莱姆病由螺旋体伯氏疏螺旋体感染引起，是北美最常见的媒介传播疾病。伯氏疏螺旋体分离株具有广泛的基因组和蛋白质组变异性，进一步比较分离株是了解螺旋体感染性和已鉴定序列变异的生物学影响的关键。在此，我们应用转录组分析和基于质谱的蛋白质组学来组装伯氏疏螺旋体实验室分离株B31、MM1和感染性分离株B31-5A4的肽数据集，以提供一个公开可用的伯氏疏螺旋体肽图集。其中包括各个分离株的总蛋白质组、分泌蛋白质组和膜蛋白质组鉴定。从35个不同实验数据集中收集的蛋白质组数据，总共进行了386次质谱运行，已鉴定出81,967个不同的肽，这些肽对应于1,113种蛋白质。伯氏疏螺旋体肽图集覆盖了1,291个蛋白质序列的B31总蛋白质组的86%。伯氏疏螺旋体肽图集是一个可扩展的综合肽库，包含来自伯氏疏螺旋体分离株的蛋白质组信息，对莱姆病研究很有用。
