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犊牛肺切片培养物对H5N1流感病毒的易感性。

Susceptibility of calf lung slice cultures to H5N1 influenza virus.

作者信息

Guilfoyle Kate, Mirolo Monica, van Amerongen Geert, van der Net Guido, Lombardo Mara Sophie, Störk Theresa, Rimmelzwaan Guus, Ludlow Martin, Osterhaus Albert

机构信息

Preclinical Speciality Services, Cerba Research (formerly Viroclinics Xplore), Schaijk, The Netherlands.

Research Center for Emerging Infection and zoonoses, University of Veterinary Medicine Hannover, Hannover, Germany.

出版信息

Emerg Microbes Infect. 2025 Dec;14(1):2432368. doi: 10.1080/22221751.2024.2432368. Epub 2024 Dec 9.

DOI:10.1080/22221751.2024.2432368
PMID:39624917
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11632935/
Abstract

The current outbreak of HPAI H5N1 virus infections in dairy cattle in the USA underscores the need for easily accessible methods to rapidly assess host susceptibility for infection with known and emerging influenza viruses. Here we show that lung slice cultures from calves provide a useful method to rapidly screen host susceptibility to a range of influenza A viruses.

摘要

美国目前爆发的高致病性禽流感H5N1病毒感染奶牛疫情凸显了对易于获取的方法的需求，以便快速评估宿主对已知和新出现的流感病毒感染的易感性。在此，我们表明，来自小牛的肺切片培养物提供了一种有用的方法，可快速筛选宿主对一系列甲型流感病毒的易感性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe4e/11632935/99f1ec0be58e/TEMI_A_2432368_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe4e/11632935/99f1ec0be58e/TEMI_A_2432368_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe4e/11632935/99f1ec0be58e/TEMI_A_2432368_F0001_OC.jpg

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本文引用的文献

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H5N1 clade 2.3.4.4b dynamics in experimentally infected calves and cows.H5N1进化分支2.3.4.4b在实验感染小牛和奶牛中的动态变化
Nature. 2025 Jan;637(8047):903-912. doi: 10.1038/s41586-024-08063-y. Epub 2024 Sep 25.
2
Genomic characterization of highly pathogenic avian influenza A H5N1 virus newly emerged in dairy cattle.奶牛中新出现的高致病性禽流感 A(H5N1)病毒的基因组特征
Emerg Microbes Infect. 2024 Dec;13(1):2380421. doi: 10.1080/22221751.2024.2380421. Epub 2024 Jul 24.
3
Pathogenicity and transmissibility of bovine H5N1 influenza virus.
牛源 H5N1 流感病毒的致病性和传染性。
Nature. 2024 Sep;633(8029):426-432. doi: 10.1038/s41586-024-07766-6. Epub 2024 Jul 8.
4
H5N1 clade 2.3.4.4b avian influenza viruses replicate in differentiated bovine airway epithelial cells cultured at air-liquid interface.H5N1 分支 2.3.4.4b 禽流感病毒在气液界面培养的分化牛气道上皮细胞中复制。
J Gen Virol. 2024 Jun;105(6). doi: 10.1099/jgv.0.002007.
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Air-liquid interface cultures of the healthy and diseased human respiratory tract: promises, challenges and future directions.健康与患病人类呼吸道的气液界面培养：前景、挑战与未来方向。
Adv Nanobiomed Res. 2021 May 6;1(6). doi: 10.1002/anbr.202000111. eCollection 2021 Jun.
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Precision-cut bovine udder slices (PCBUS) as an in-vitro-model of an early phase of infection of bovine mastitis.精确切割的牛乳房切片（PCBUS）作为牛乳腺炎早期感染的体外模型。
BMC Vet Res. 2021 Mar 16;17(1):120. doi: 10.1186/s12917-021-02817-w.
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J Virol Methods. 2013 Oct;193(1):159-65. doi: 10.1016/j.jviromet.2013.06.016. Epub 2013 Jun 15.
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