Kato Hiromichi, Ambai Shota, Ikeda Fumiya, Abe Koji, Nakamura Satoshi, Yatsunami Rie
School of Life Science and Technology, Institute of Science Tokyo, 4259 Nagatsuta, Midori-ku, Yokohama, 226-8501, Japan.
Extremophiles. 2024 Dec 3;29(1):7. doi: 10.1007/s00792-024-01370-2.
The novel esterase gene lipP1, which encodes HjEstP1, was discovered in the genome of the extremely halophilic archaeon Haloarcula japonica. A homology search and sequence alignment revealed that HjEstP1 is a member of family IV esterases with conserved GXSXG and HGGG motifs. lipP1 was expressed in its parental strain, and recombinant HjEstP1 was purified and characterized. Optimal pH and temperature of HjEstP1 were 6.0 and > 60 °C, respectively. HjEstP1 showed higher activity with increasing NaCl concentration, and optimal NaCl concentration was > 4.5 M. Furthermore, HjEstP1 preferentially hydrolyzed pNP and glycerol esters with short chain fatty acids. To our knowledge, this is the first report of an esterase from an extremely halophilic archaeon obtained via homologous expression.
在极端嗜盐古菌日本嗜盐栖热菌（Haloarcula japonica）的基因组中发现了编码HjEstP1的新型酯酶基因lipP1。同源性搜索和序列比对显示，HjEstP1是具有保守GXSXG和HGGG基序的IV型酯酶家族成员。lipP1在其亲本菌株中表达，重组HjEstP1被纯化并进行了表征。HjEstP1的最适pH和温度分别为6.0和>60°C。随着NaCl浓度的增加，HjEstP1表现出更高的活性，最适NaCl浓度>4.5 M。此外，HjEstP1优先水解含有短链脂肪酸的对硝基苯酯（pNP）和甘油酯。据我们所知，这是通过同源表达从极端嗜盐古菌中获得酯酶的首次报道。
