Adameyko Igor, Bakken Trygve, Bhaduri Aparna, Chhatbar Chintan, Filbin Mariella G, Gate David, Hochgerner Hannah, Kim Chang Nam, Krull Jordan, La Manno Gioele, Li Qingyun, Linnarsson Sten, Ma Qin, Mayer Christian, Menon Vilas, Nano Patricia, Prinz Marco, Quake Steve, Walsh Christopher A, Yang Jin, Bayraktar Omer Ali, Gokce Ozgun, Habib Naomi, Konopka Genevieve, Liddelow Shane A, Nowakowski Tomasz J
Department of Neuroimmunology, Center for Brain Research, Medical University of Vienna, Vienna, Austria.
Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden.
Nat Neurosci. 2024 Dec;27(12):2278-2291. doi: 10.1038/s41593-024-01827-9. Epub 2024 Dec 3.
Single-cell and single-nucleus genomic approaches can provide unbiased and multimodal insights. Here, we discuss what constitutes a molecular cell atlas and how to leverage single-cell omics data to generate hypotheses and gain insights into cell transitions in development and disease of the nervous system. We share points of reflection on what to consider during study design and implementation as well as limitations and pitfalls.
单细胞和单细胞核基因组方法能够提供无偏差的多模态见解。在此，我们将探讨什么构成分子细胞图谱，以及如何利用单细胞组学数据来生成假设并深入了解神经系统发育和疾病中的细胞转变。我们分享在研究设计和实施过程中需要考虑的要点，以及局限性和陷阱。
