National Research Council of Italy (CNR)-Institute of Protein Biochemistry (IBP), Naples, Italy.
Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, FL, USA.
Mar Biotechnol (NY). 2018 Aug;20(4):502-511. doi: 10.1007/s10126-018-9821-9. Epub 2018 Apr 12.
The exploration of poorly studied areas of Earth can highly increase the possibility to discover novel bioactive compounds. In this study, the cultivable fraction of fungi and bacteria from Barents Sea sediments has been studied to mine new bioactive molecules with antibacterial activity against a panel of human pathogens. We isolated diverse strains of psychrophilic and halophilic bacteria and fungi from a collection of nine samples from sea sediment. Following a full bioassay-guided approach, we isolated a new promising polyextremophilic marine fungus strain 8Na, identified as Aspergillus protuberus MUT 3638, possessing the potential to produce antimicrobial agents. This fungus, isolated from cold seawater, was able to grow in a wide range of salinity, pH and temperatures. The growth conditions were optimised and scaled to fermentation, and its produced extract was subjected to chemical analysis. The active component was identified as bisvertinolone, a member of sorbicillonoid family that was found to display significant activity against Staphylococcus aureus with a minimum inhibitory concentration (MIC) of 30 μg/mL.
对地球研究较少的领域的探索极有可能发现新的具有生物活性的化合物。在这项研究中，对巴伦支海沉积物中的可培养真菌和细菌进行了研究，以挖掘具有抗菌活性的新型生物活性分子，针对一组人类病原体。我们从九份海洋沉积物样本中分离出了多种嗜冷和嗜盐细菌和真菌。经过全面的生物活性导向分离方法，我们从一株新的有前景的海洋多极端真菌菌株 8Na 中分离出了一种新型多极端海洋真菌菌株 8Na，鉴定为 Aspergillus protuberus MUT 3638，具有产生抗菌剂的潜力。该真菌从冷海水中分离出来，能够在广泛的盐度、pH 值和温度范围内生长。对其生长条件进行了优化和放大到发酵，并对其产生的提取物进行了化学分析。活性成分被鉴定为双倍他醇酮，这是一种属于索比醇类的化合物，对金黄色葡萄球菌显示出显著的活性，最小抑菌浓度 (MIC) 为 30 μg/mL。
