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Selection of reference genes for quantitative real-time PCR analysis in exogenous hormone-treated .

作者信息

Zhao Wei, Tian Ying, Wang Yi, Wu Yun, Ren Ziming, Li Kehu

机构信息

Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Collaborative Innovation Center for Mountain Ecology & Agro-Bioengineering (CICMEAB), Institute of Agro-Bioengineering, College of Life Sciences, Guizhou University, Guiyang 550025, China.

College of Horticulture Science, Zhejiang A&F University, Hangzhou 311300, China.

出版信息

Acta Biochim Biophys Sin (Shanghai). 2024 Dec 4;57(4):671-675. doi: 10.3724/abbs.2024197.

DOI:10.3724/abbs.2024197
PMID:39632660
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12040745/
Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bfe4/12040745/5bd4663caa23/abbs-2024-593-t2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bfe4/12040745/fa5ed56a86cc/abbs-2024-593-t1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bfe4/12040745/5bd4663caa23/abbs-2024-593-t2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bfe4/12040745/fa5ed56a86cc/abbs-2024-593-t1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bfe4/12040745/5bd4663caa23/abbs-2024-593-t2.jpg

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本文引用的文献

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Nat Prod Rep. 2019 Oct 16;36(10):1462-1488. doi: 10.1039/c8np00055g.
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Alkaloids from Lycoris aurea and their cytotoxicities against the head and neck squamous cell carcinoma.黄花石蒜中的生物碱及其对头颈部鳞状细胞癌的细胞毒性。
Fitoterapia. 2014 Jun;95:121-6. doi: 10.1016/j.fitote.2014.03.006. Epub 2014 Mar 14.
3
miRDeepFinder: a miRNA analysis tool for deep sequencing of plant small RNAs.miRDeepFinder：一种用于植物小RNA深度测序的miRNA分析工具。
Plant Mol Biol. 2012 Jan 31. doi: 10.1007/s11103-012-9885-2.
4
Evaluation of suitable reference genes for gene expression studies in Lycoris longituba.长筒石蒜基因表达研究中合适内参基因的评估
J Genet. 2011 Dec;90(3):503-6. doi: 10.1007/s12041-011-0113-7.
5
Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets.实时定量逆转录-PCR数据的标准化：一种基于模型的方差估计方法，用于鉴定适合标准化的基因，并应用于膀胱癌和结肠癌数据集。
Cancer Res. 2004 Aug 1;64(15):5245-50. doi: 10.1158/0008-5472.CAN-04-0496.
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Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper--Excel-based tool using pair-wise correlations.稳定管家基因、差异调节靶基因及样本完整性的测定：BestKeeper - 基于Excel的采用成对相关性分析的工具。
Biotechnol Lett. 2004 Mar;26(6):509-15. doi: 10.1023/b:bile.0000019559.84305.47.
7
Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems.使用实时逆转录聚合酶链反应（RT-PCR）对mRNA进行定量分析：趋势与问题。
J Mol Endocrinol. 2002 Aug;29(1):23-39. doi: 10.1677/jme.0.0290023.
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Genome Biol. 2002 Jun 18;3(7):RESEARCH0034. doi: 10.1186/gb-2002-3-7-research0034.