通过聚丙烯酰胺凝胶电泳纯化小寡核苷酸并转移至二乙氨基乙基纸上。
Purification of small oligonucleotides by polyacrylamide gel electrophoresis and transfer to diethylaminoethyl paper.
作者信息
Vorndam A V, Kerschner J
出版信息
Anal Biochem. 1986 Feb 1;152(2):221-5. doi: 10.1016/0003-2697(86)90401-x.
PMID:3963359
Abstract
The application of polyacrylamide gel electrophoresis with subsequent electroelution onto DEAE paper for the purification of small oligonucleotides is described. We demonstrate that synthetic DNAs and hydrolyzed RNAs as small as three nucleotides in length can be purified by this technique. The product is undegraded and homogeneous in length.
摘要
描述了应用聚丙烯酰胺凝胶电泳并随后电洗脱到DEAE纸上以纯化小寡核苷酸的方法。我们证明，长度小至三个核苷酸的合成DNA和水解RNA可以通过该技术纯化。产物未降解且长度均一。
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