Chou Chia-Hua, Chao Qiao-Ting, Lu Yun-Shan, Lee Tai-Fen, Hsueh Po-Ren, Huang Yu-Tsung, Liao Chun-Hsing
Department of Laboratory Medicine, National Taiwan University Hospital, Taipei, Taiwan.
Division of Infectious Diseases, Department of Internal Medicine, China Medical University, School of Medicine, Taichung, Taiwan.
J Clin Microbiol. 2025 Jan 31;63(1):e0104824. doi: 10.1128/jcm.01048-24. Epub 2024 Dec 5.
BluePoint MoldID can identify 43 fungal species through nucleic acid array hybridization and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) can identify 247 filamentous fungi through mass spectrometry. First, 43 standard isolates from the Bioresource Collection and Research Center, Taiwan, and the College of American Pathologists and 41 clinical species isolates confirmed by rDNA-ITS sequencing were analyzed using BluePoint MoldID and Bruker MALDI-TOF MS. BluePoint MoldID accurately identified 79% (34/43) of the standard isolates to the species level but failed to recognize nine isolates absent from its database; additionally, 87.8% (36/41) of the clinical isolates were identified at the species level, with 80.5% (33/41) accuracy. MALDI-TOF MS identified 86% (37/43) of the standard isolates, with 81.4% (35/43) accurately identified at the species level and two at the genus level, and identified all the clinical isolates, with 92.6% (38/41) accurately identified at the species level. Next, we analyzed 93 clinical species and compared the results by rDNA-ITS sequencing. BluePoint MoldID identified 87.1% (81/93) of the isolates at the species level, with 80.6% (75/93) accuracy. MALDI-TOF MS identified 97.8% (91/93) of the isolates, including some uncommon species, with 90.3% (84/93) accuracy at the species level. BluePoint MoldID and MALDI-TOF MS had turnaround times of 8 and 2 h, respectively, significantly reducing the time needed to identify filamentous fungi.
The BluePoint MoldID kit is an oligonucleotide array used for the identification of filamentous fungi, and it has not yet been mentioned in recent studies. We used a BluePoint MoldID kit to identify standard and clinical filamentous fungal isolates and compared its performance with that of Bruker MALDI-TOF MS. The former accurately identified 80.2% of the isolates (142/177), and the latter identified 92.6% of the isolates (164/177). The performance of the BluePoint MoldID kit was slightly inferior to that of Bruker MALDI-TOF MS because of the smaller database. However, the BluePoint MoldID kit can cover most clinically common opportunistic fungal infections; thus, it offers an alternative method for laboratories that lack MALDI-TOF MS equipment, as the device is less expensive.
BluePoint MoldID可通过核酸阵列杂交鉴定43种真菌，基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）可通过质谱鉴定247种丝状真菌。首先，使用BluePoint MoldID和布鲁克MALDI-TOF MS对来自台湾生物资源保存及研究中心、美国病理学家学会的43株标准菌株以及经rDNA-ITS测序确认的41株临床分离菌株进行分析。BluePoint MoldID能将79%（34/43）的标准菌株准确鉴定到种水平，但未能识别其数据库中没有的9株菌株；此外，87.8%（36/41）的临床分离菌株被鉴定到种水平，准确率为80.5%（33/41）。MALDI-TOF MS鉴定出86%（37/43）的标准菌株，其中种水平准确鉴定率为81.4%（35/43），属水平鉴定出2株，且鉴定出了所有临床分离菌株，种水平准确鉴定率为92.6%（38/41）。接下来，我们分析了93株临床菌株，并通过rDNA-ITS测序比较结果。BluePoint MoldID将87.1%（81/93）的分离菌株鉴定到种水平，准确率为80.6%（75/93）。MALDI-TOF MS鉴定出97.8%（91/93）的分离菌株，包括一些罕见菌种，种水平准确率为90.3%（84/93）。BluePoint MoldID和MALDI-TOF MS的周转时间分别为8小时和2小时，显著缩短了鉴定丝状真菌所需的时间。
BluePoint MoldID试剂盒是一种用于鉴定丝状真菌的寡核苷酸阵列，近期研究中尚未提及。我们使用BluePoint MoldID试剂盒鉴定标准和临床丝状真菌分离菌株，并将其性能与布鲁克MALDI-TOF MS的性能进行比较。前者准确鉴定了80.2%的分离菌株（142/177），后者鉴定了92.6%的分离菌株（164/177）。由于数据库较小，BluePoint MoldID试剂盒的性能略逊于布鲁克MALDI-TOF MS。然而，BluePoint MoldID试剂盒可以覆盖大多数临床上常见的机会性真菌感染；因此，对于缺乏MALDI-TOF MS设备的实验室来说，它提供了一种替代方法，因为该设备价格较低。
