Wu Qing-Juan, Chen Lan-Yu, Sun Quan-Mei, Wang Ning, Han Dong, Lv Wen-Liang
Guang 'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing, PR China.
Natl Ctr Nanosci & Technol, CAS Ctr Excellence Nanosci, Beijing, PR China.
Photoacoustics. 2024 Nov 14;41:100667. doi: 10.1016/j.pacs.2024.100667. eCollection 2025 Feb.
BACKGROUND & AIMS: Effective anti-fibrotic drugs and new non-invasive evaluation methods for liver fibrosis (LF) are urgently needed. Our study aimed to evaluate the histological effects of the Qizhu (QZ) formula on LF and to explore a non-invasive Near-infrared photoacoustic imaging (NIR-PAI) kinetic model for liver function detection and pharmacodynamic evaluation.
C57BL/6 J mice were randomly divided into six groups (n=6). An LF model was induced by CCl for 8 weeks, followed by an 8-week treatment period. Histological and serological parameters were assessed, and indocyanine green (ICG) metabolism (maximum peak time [T] and half-life [T]) was monitored by NIR-PAI. Spearman correlation analysis was conducted to evaluate correlations.
RESULTS & CONCLUSIONS: Histological and serological results confirmed the anti-fibrotic effects of QZ. NIR-PAI kinetic parameters indicated that QZ shortened the T and T of ICG. There were good correlations between ICG metabolism and liver histopathology. The non-invasive NIR-PAI kinetic model shows potential in liver function detection and pharmacodynamic evaluation.
迫切需要有效的抗纤维化药物以及用于肝纤维化（LF）的新型非侵入性评估方法。我们的研究旨在评估芪术（QZ）配方对肝纤维化的组织学影响，并探索一种用于肝功能检测和药效学评估的非侵入性近红外光声成像（NIR-PAI）动力学模型。
将C57BL/6 J小鼠随机分为六组（n = 6）。用四氯化碳诱导肝纤维化模型8周，随后进行为期8周的治疗期。评估组织学和血清学参数，并通过近红外光声成像监测吲哚菁绿（ICG）代谢（最大峰值时间[T]和半衰期[T]）。进行Spearman相关性分析以评估相关性。
组织学和血清学结果证实了芪术的抗纤维化作用。近红外光声成像动力学参数表明芪术缩短了吲哚菁绿的T和T。吲哚菁绿代谢与肝脏组织病理学之间存在良好的相关性。非侵入性近红外光声成像动力学模型在肝功能检测和药效学评估中显示出潜力。
