Zhu Jiang, Sun Yue, Ma Lingyan, Chen Qu, Hu Caihong, Yang Hua, Hong Qihua, Xiao Yingping
College of Animal Sciences, Zhejiang University, Key Laboratory of Molecular Animal Nutrition (Zhejiang University), Ministry of Education, Hangzhou 310058, China.
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro -products, Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
Anim Nutr. 2024 Sep 28;19:401-410. doi: 10.1016/j.aninu.2024.05.013. eCollection 2024 Dec.
Diarrhea poses a significant threat to the health and well-being of weaned piglets, leading to substantial morbidity and mortality and economic loss in the pig industry. However, the structural characteristics of the gut microbiota and the key genera associated with early diarrhea in piglets within large-scale production systems are poorly understood. This study aimed to investigate the differences in the microbial community structure and the specific genera alteration between the healthy piglets and diarrhea piglets, and to identify the biomarkers of gut microbiota associated with diarrhea in piglets. A total of 250 fecal samples, including 130 healthy piglets (Duroc × Landrace × Large Yorkshire) in the Control group and 120 from diarrhea piglets in Diarrhea group, were collected from three large-scale farms as discovery cohorts and were used for 16S rRNA gene sequencing. Additionally, 150 fecal samples from another large-scale pig farm were collected for the validation trail. The Chao1 and ACE indices were obviously lower ( < 0.01) in the diarrhea piglets compared to the healthy ones. Principal coordinate analysis showed significant differences in the distance matrix of gut microbiota between the healthy and diarrhea piglets (Bray-Curtis: = 0.001, Jaccard: = 0.001). Eighty-five genera were differentially enriched ( < 0.001) between healthy and diarrhea piglets. Notably, , , , , and were identified as potential biomarkers of diarrhea susceptibility; , , , , and were found to be beneficial for maintaining intestinal homeostasis. These differentially enriched genera of healthy and diarrhea piglets were further confirmed in the validation cohort. In conclusion, this study identified the diarrhea-associated and beneficial genera in the faces of piglet, providing a theoretical basis for the diagnosis and intervention of diarrhea in weaned piglets.
腹泻对断奶仔猪的健康和福祉构成重大威胁，导致养猪业出现大量发病和死亡情况以及经济损失。然而，大规模生产系统中仔猪肠道微生物群的结构特征以及与早期腹泻相关的关键属知之甚少。本研究旨在调查健康仔猪和腹泻仔猪之间微生物群落结构的差异以及特定属的变化，并确定与仔猪腹泻相关的肠道微生物群生物标志物。从三个大型农场收集了总共250份粪便样本作为发现队列，其中包括对照组的130头健康仔猪（杜洛克×长白×大白）和腹泻组的120头腹泻仔猪粪便样本，并用于16S rRNA基因测序。此外，从另一个大型养猪场收集了150份粪便样本用于验证试验。与健康仔猪相比，腹泻仔猪的Chao1和ACE指数明显较低（<0.01）。主坐标分析显示健康仔猪和腹泻仔猪肠道微生物群的距离矩阵存在显著差异（Bray-Curtis：=0.001，Jaccard：=0.001）。健康仔猪和腹泻仔猪之间有85个属差异富集（<0.001）。值得注意的是，、、、、和被确定为腹泻易感性的潜在生物标志物；、、、、和被发现有利于维持肠道稳态。这些健康仔猪和腹泻仔猪中差异富集的属在验证队列中得到进一步证实。总之，本研究确定了仔猪粪便中与腹泻相关的属和有益属，为断奶仔猪腹泻的诊断和干预提供了理论依据。
