流式细胞术检测睾丸癌中c-myc癌蛋白定量的临床意义
The clinical significance of flow cytometric c-myc oncoprotein quantitation in testicular cancer.
作者信息
Watson J V, Stewart J, Evan G I, Ritson A, Sikora K
出版信息
Br J Cancer. 1986 Mar;53(3):331-7. doi: 10.1038/bjc.1986.56.
Abstract
A sensitive flow cytometric assay has been developed using a monoclonal antibody, Myc 1-6E10, to quantitate c-myc oncoprotein levels in nuclei isolated from wax embedded testicular tumours. The oncoprotein (p62c-myc) level increased significantly with increasing teratoma differentiation. Patients with intermediate and undifferentiated tumours who developed recurrence had lower p62c-myc levels than those who were disease free since their initial treatment. Such quantitative biochemical methods may provide new prognostic indices for cancer patients.
摘要
已开发出一种灵敏的流式细胞术检测方法，使用单克隆抗体Myc 1-6E10来定量从石蜡包埋的睾丸肿瘤中分离出的细胞核中的c-myc癌蛋白水平。随着畸胎瘤分化程度的增加，癌蛋白（p62c-myc）水平显著升高。发生复发的中度和未分化肿瘤患者的p62c-myc水平低于自初始治疗后无疾病的患者。此类定量生化方法可能为癌症患者提供新的预后指标。
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