Dencher N A
Biochemistry. 1986 Mar 11;25(5):1195-200. doi: 10.1021/bi00353a038.
Functional reconstitution of the membrane protein bacteriorhodopsin into lipid vesicles is achieved by mixing aqueous suspensions of long-chain lecithins and purple membrane with the short-chain lecithin diheptanoylphosphatidylcholine (20 mol % of total lipid). The membrane protein is transmembranously inserted in the lipid bilayer of the vesicle and highly active as a light-energized proton pump. This rapid, easy, and gentle procedure might allow functional reconstitution of other membrane systems and isolated membrane proteins as well.
通过将长链卵磷脂和紫膜的水悬浮液与短链卵磷脂二庚酰磷脂酰胆碱（占总脂质的20摩尔%）混合，可实现膜蛋白细菌视紫红质在脂质囊泡中的功能重建。该膜蛋白跨膜插入囊泡的脂质双层中，并作为光驱动质子泵具有高活性。这种快速、简便且温和的方法可能也适用于其他膜系统和分离的膜蛋白的功能重建。
