Yang Binghui, Ma Jinlong, Gu Hui, Xu Yunshu, Long Mingxin, Xu Tiantian, Liu Mingzhi, Yin Heng, Xu Qingsong
Key Laboratory of Biotechnology and Bioresources Utilization of Ministry of Education, Dalian Minzu University, Dalian 116600, China.
Dalian Engineering Research Center for Carbohydrate Agricultural Preparations, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Int J Biol Macromol. 2025 Jan;286:138513. doi: 10.1016/j.ijbiomac.2024.138513. Epub 2024 Dec 6.
To explore the immunomodulatory activity of polysaccharides from Ampelopsis grossedentata, two polysaccharides named AGP1 and AGP2 were isolated and purified by DEAE-cellulose 52 column and Sephacryl S-300HR chromatography. AGP1 and AGP2 were composed of fucose, arabinose, rhamnose, galactose, glucose, mannose, galacturonic acid, and glucuronic acid, with a ratio of 0.5: 10.2: 0.9: 31.8: 7.4: 3.4: 21.6: 24.2 and 0.4: 6.0: 0.5: 23.3: 3.3: 6.2: 33.5: 26.8, respectively. The average molecular weights of AGP1 and AGP2 were found to be 6.60 × 10 Da and 7.24 × 10 Da, respectively. AGP1 contained →4,6)-Galp-(1 → glycosidic linkages, while AGP2 contained →2)-Galp-(1 → and →2,3,4)-Glcp-(1 → glycosidic linkages. The structures of AGPs were characterized by Fourier transform infrared spectroscopy, nuclear magnetic resonance, and scanning electron microscope. The immunomodulatory activity of AGPs was investigated in RAW264.7 cells, and the results indicated that AGPs significantly activated macrophages, promoted cells differentiation and NO secretion, increased the expression of IL-6 and TNF-α, and induced macrophage M1 polarization. Transcriptomic analysis indicated that AGP1 and AGP2 regulated a total of 1043 and 970 differentially expressed genes respectively, which were identified in different immune related signaling pathways. Moreover, the immunoblot demonstrated that AGPs exerted immune-promoting effects through the TLR4, MAPK and NF-κB signaling pathways in macrophages. Consequently, AGPs have potent immunomodulatory activity and can be considered as immunomodulators in medical and food industries.
为探究显齿蛇葡萄多糖的免疫调节活性，通过DEAE-纤维素52柱和Sephacryl S-300HR色谱法分离纯化得到两种多糖，命名为AGP1和AGP2。AGP1和AGP2由岩藻糖、阿拉伯糖、鼠李糖、半乳糖、葡萄糖、甘露糖、半乳糖醛酸和葡萄糖醛酸组成，其比例分别为0.5:10.2:0.9:31.8:7.4:3.4:21.6:24.2和0.4:6.0:0.5:23.3:3.3:6.2:33.5:26.8。AGP1和AGP2的平均分子量分别为6.60×10 Da和7.24×10 Da。AGP1含有→4,6)-Galp-(1→糖苷键，而AGP2含有→2)-Galp-(1→和→2,3,4)-Glcp-(1→糖苷键。通过傅里叶变换红外光谱、核磁共振和扫描电子显微镜对AGP的结构进行了表征。在RAW264.7细胞中研究了AGP的免疫调节活性，结果表明AGP显著激活巨噬细胞，促进细胞分化和NO分泌，增加IL-6和TNF-α的表达，并诱导巨噬细胞M1极化。转录组分析表明，AGP1和AGP2分别调控了1043个和970个差异表达基因，这些基因在不同的免疫相关信号通路中被鉴定出来。此外，免疫印迹表明AGP通过巨噬细胞中的TLR4、MAPK和NF-κB信号通路发挥免疫促进作用。因此，AGP具有强大的免疫调节活性，可被视为医药和食品工业中的免疫调节剂。
