Arévalo-Jaimes Betsy Verónica, Torrents Eduard
Bacterial infections and antimicrobial therapies group, Institute for Bioengineering of Catalonia (IBEC), Baldiri Reixac Street 10, 08037, Barcelona, Spain.
Microbiology Section, Department of Genetics, Microbiology and Statistics, Faculty of Biology, University of Barcelona, Diagonal Street 647, 08028, Barcelona, Spain.
Curr Res Microb Sci. 2024 Nov 17;7:100316. doi: 10.1016/j.crmicr.2024.100316. eCollection 2024.
and have been co-isolated from several biofilm-associated diseases, including those related to medical devices. This association confers advantages to both microorganisms, resulting in detrimental effects on the host. To elucidate this phenomenon, the present study investigated colony changes derived from non-physical interactions between and . We performed proximity assays by confronting colonies of the yeast and the bacteria on agar plates at six different distances for 9-10 days. We found that colony variants of originated progressively after prolonged exposure to proximity, specifically in response to pH neutralization of the media by the fungi. The new phenotypes of were more virulent in a larvae model compared to colonies grown without influence. This event was associated with an upregulation of RNA III and a expression, suggesting a role for α-toxin. Our findings indicate that enhances virulence by inducing the formation of more aggressive colonies. This highlights the importance of understanding the intricate connection between environmental responses, virulence and, fitness in pathogenesis.
并且已从几种与生物膜相关的疾病中共同分离出来，包括与医疗设备相关的疾病。这种关联赋予了两种微生物优势，对宿主产生有害影响。为了阐明这一现象，本研究调查了由[具体微生物1]和[具体微生物2]之间的非物理相互作用产生的菌落变化。我们通过在琼脂平板上以六种不同距离将酵母和细菌的菌落对峙9至10天来进行接近度测定。我们发现，在长时间暴露于[具体微生物2]接近后，[具体微生物1]的菌落变体逐渐出现，特别是对真菌使培养基pH值中和的反应。与在没有[具体微生物2]影响下生长的菌落相比，[具体微生物1]的新表型在蜡螟幼虫模型中更具毒性。这一事件与RNA III和[具体基因]表达的上调有关，表明α-毒素起了作用。我们的研究结果表明，[具体微生物2]通过诱导形成更具侵袭性的菌落来增强[具体微生物1]的毒力。这突出了理解环境反应、毒力和[具体微生物1]发病机制中适应性之间复杂联系的重要性。
