Khine Nwai Oo, Shah Asad Ali, Chatsuwan Tanittha, Yindee Jitrapa, Supimon Natthapong, Saenkankam Imporn, Hampson David John, Prapasarakul Nuvee
Department of Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
Front Vet Sci. 2024 Nov 25;11:1464934. doi: 10.3389/fvets.2024.1464934. eCollection 2024.
Carbapenem-resistant Enterobacterales (CRE), particularly carbapenemase-producing , pose a significant global health challenge due to their resistance to last-resort antibiotics. This study investigates the genetic characteristics and clonal relationships of CRE isolated from canine and human clinical samples in Bangkok to understand potential interspecies transmission.
Fifty-two CRE isolates were collected from 477 clinical samples from dogs and humans at Chulalongkorn University between 2017-2021. Bacterial species were identified using MALDI-TOF, and antimicrobial resistance was confirmed through broth microdilution testing. Genetic analyses included plasmid replicon typing, multilocus sequence typing (MLST), whole genome sequencing (WGS), and pulsed-field gel electrophoresis (PFGE) to assess resistance genes and clonal relatedness.
CRE isolates from both species exhibited genetic variability with high ARG counts, particularly in human isolates. MLST identified ST410 in most E. coli isolates from both dogs and humans, and IncFIA/IncFIB plasmids were predominant among blaNDM-positive isolates. PFGE patterns and SNP analysis showed no clonal relationship between canine and human isolates, suggesting independent acquisition pathways for CRE in the two hosts.
The study highlights the absence of direct clonal transmission between canine and human isolates but reveals overlapping sequence types and plasmid types. The findings underscore the potential for interspecies transmission under certain conditions, emphasizing the importance of a One Health approach for monitoring CRE in both human and animal populations.
耐碳青霉烯类肠杆菌科细菌（CRE），尤其是产碳青霉烯酶的细菌，由于其对最后手段抗生素具有耐药性，对全球健康构成了重大挑战。本研究调查了从曼谷犬类和人类临床样本中分离出的CRE的遗传特征和克隆关系，以了解潜在的种间传播情况。
2017年至2021年期间，从朱拉隆功大学的477份犬类和人类临床样本中收集了52株CRE分离株。使用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF）鉴定细菌种类，并通过肉汤微量稀释试验确认抗菌药物耐药性。遗传分析包括质粒复制子分型、多位点序列分型（MLST）、全基因组测序（WGS）和脉冲场凝胶电泳（PFGE），以评估耐药基因和克隆相关性。
两个物种的CRE分离株均表现出遗传变异性，耐药基因数量较多，尤其是在人类分离株中。MLST在大多数犬类和人类大肠杆菌分离株中鉴定出ST410，IncFIA/IncFIB质粒在blaNDM阳性分离株中占主导地位。PFGE图谱和单核苷酸多态性（SNP）分析表明，犬类和人类分离株之间没有克隆关系，这表明两种宿主中的CRE获得途径是独立的。
该研究强调了犬类和人类分离株之间不存在直接的克隆传播，但揭示了重叠的序列类型和质粒类型。这些发现强调了在某些条件下种间传播的可能性，强调了采用“同一健康”方法监测人类和动物群体中CRE的重要性。
