Boško Rastislav, Pluháčková Helena, Martiník Jan, Benešová Karolína, Svoboda Zdeněk, Běláková Sylvie, Pernica Marek
Research Institute of Brewing and Malting, Mostecká 7, 614 00, Brno, Czech Republic.
Faculty of AgriSciences, Department of Crop Science, Breeding and Plant Medicine, Mendel University in Brno, Zemědělská 1, 613 00, Brno, Czech Republic.
Mycotoxin Res. 2025 Feb;41(1):199-206. doi: 10.1007/s12550-024-00577-8. Epub 2024 Dec 10.
The silymarin complex extracted from milk thistle provides significant health benefits, particularly due to its antioxidant and hepatoprotective properties. However, plant substances can be contaminated by a number of fungi types and their secondary metabolites-mycotoxins. This work deals with the determination of aflatoxins and zearalenone and its metabolites in 39 different samples grown in 2020 and 2021. Analysis of mycotoxins was performed by UHPLC-MS/MS after immunoaffinity column AFLAPREP and EASI-EXTRACT ZEARALENONE clean-up. The presence of aflatoxins was not confirmed in the monitored samples, but 1/3 of the samples were contaminated with zearalenone in the range of 2.8-378.9 µg/kg. Metabolites of zearalenone such as α-zearalenol, α-zearalanol, and β-zearalanol were not detected in any of the samples. β-Zearalenol was found in two samples (2.6 µg/kg and 29.8 µg/kg).
从水飞蓟中提取的水飞蓟素复合物具有显著的健康益处，特别是因其抗氧化和保肝特性。然而，植物物质可能会被多种真菌及其次生代谢产物——霉菌毒素污染。这项工作涉及测定2020年和2021年种植的39个不同样品中的黄曲霉毒素、玉米赤霉烯酮及其代谢产物。霉菌毒素分析在免疫亲和柱AFLAPREP和EASI - EXTRACT ZEARALENONE净化后，通过超高效液相色谱 - 串联质谱法（UHPLC - MS/MS）进行。在所监测的样品中未确认存在黄曲霉毒素，但三分之一的样品被玉米赤霉烯酮污染，污染范围为2.8 - 378.9微克/千克。在任何样品中均未检测到玉米赤霉烯酮的代谢产物，如α - 玉米赤霉醇、α - 玉米赤霉烯醇和β - 玉米赤霉烯醇。在两个样品中发现了β - 玉米赤霉烯醇（2.6微克/千克和29.8微克/千克）。
