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MVL3(一种非裂解性杀细胞支原体病毒)感染莱氏无胆甾原体期间的病毒和宿主细胞DNA合成

Virus and host cell DNA syntheses during infection of Acholeplasma laidlawii by MVL3, a nonlytic cytocidal mycoplasmavirus.

作者信息

Haberer K, Maniloff J

出版信息

J Virol. 1980 Feb;33(2):671-9. doi: 10.1128/JVI.33.2.671-679.1980.

Abstract

The replication of mycoplasmavirus MVL3 in Acholeplasma laidlawii K2 host cells was studied by analysis of infected-cell lysates using sedimentation in sucrose gradients and DNA-DNA hybridization. Viral DNA replication was found to involve intermediates sedimenting faster than free viral DNA, which is a linear, double-stranded molecule of about 26 x 10(6) daltons. After the shutdown of cellular DNA synthesis, viral DNA synthesis continued for many hours. The fate of cellular and parental viral DNAs was examined.

摘要

通过使用蔗糖梯度沉降和DNA-DNA杂交分析感染细胞裂解物,研究了支原体病毒MVL3在莱氏无胆甾原体K2宿主细胞中的复制情况。发现病毒DNA复制涉及比游离病毒DNA沉降更快的中间体,游离病毒DNA是一种线性双链分子,分子量约为26×10⁶道尔顿。细胞DNA合成停止后,病毒DNA合成持续了许多小时。研究了细胞DNA和亲本病毒DNA的命运。

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