Haberer K, Maniloff J
J Virol. 1980 Feb;33(2):671-9. doi: 10.1128/JVI.33.2.671-679.1980.
The replication of mycoplasmavirus MVL3 in Acholeplasma laidlawii K2 host cells was studied by analysis of infected-cell lysates using sedimentation in sucrose gradients and DNA-DNA hybridization. Viral DNA replication was found to involve intermediates sedimenting faster than free viral DNA, which is a linear, double-stranded molecule of about 26 x 10(6) daltons. After the shutdown of cellular DNA synthesis, viral DNA synthesis continued for many hours. The fate of cellular and parental viral DNAs was examined.
通过使用蔗糖梯度沉降和DNA-DNA杂交分析感染细胞裂解物,研究了支原体病毒MVL3在莱氏无胆甾原体K2宿主细胞中的复制情况。发现病毒DNA复制涉及比游离病毒DNA沉降更快的中间体,游离病毒DNA是一种线性双链分子,分子量约为26×10⁶道尔顿。细胞DNA合成停止后,病毒DNA合成持续了许多小时。研究了细胞DNA和亲本病毒DNA的命运。