Cao Rui, Luo Yilin, Zhao Jingjing, Zeng Yushun, Zhang Yide, Zhou Qifa, le da Zerda Adam, Wang Lihong V
Caltech Optical Imaging Laboratory, Andrew and Peggy Cherng Department of Medical Engineering, Department of Electrical Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
Department of Structural Biology, Stanford University, Stanford, CA 94305, USA.
Sci Adv. 2024 Dec 13;10(50):eado0518. doi: 10.1126/sciadv.ado0518. Epub 2024 Dec 11.
Intraoperative imaging of slide-free specimens is crucial for oncology surgeries, allowing surgeons to quickly identify tumor margins for precise surgical guidance. While high-resolution ultraviolet photoacoustic microscopy has been demonstrated for slide-free histology, the imaging speed is insufficient, due to the low laser repetition rate and the limited depth of field. To address these challenges, we present parallel ultraviolet photoacoustic microscopy (PUV-PAM) with simultaneous scanning of eight optical foci to acquire histology-like images of slide-free fresh specimens, improving the ultraviolet PAM imaging speed limited by low laser repetition rates. The PUV-PAM has achieved an imaging speed of 0.4 square millimeters per second (i.e., 4.2 minutes per square centimeter) at 1.3-micrometer resolution using a 50-kilohertz laser. In addition, we demonstrated the PUV-PAM with eight needle-shaped beams for an extended depth of field, allowing fast imaging of slide-free tissues with irregular surfaces. We believe that the PUV-PAM approach will enable rapid intraoperative photoacoustic histology and provide prospects for ultrafast optical-resolution PAM.
术中对无玻片标本进行成像对于肿瘤手术至关重要，它能让外科医生快速识别肿瘤边缘，以获得精确的手术指导。虽然高分辨率紫外光声显微镜已被用于无玻片组织学成像，但由于激光重复频率低和景深有限，成像速度不足。为应对这些挑战，我们提出了并行紫外光声显微镜（PUV-PAM），通过同时扫描八个光学焦点来获取无玻片新鲜标本的组织学样图像，提高了受低激光重复频率限制的紫外光声显微镜成像速度。使用50千赫兹的激光，PUV-PAM在1.3微米分辨率下实现了每秒0.4平方毫米的成像速度（即每平方厘米4.2分钟）。此外，我们展示了具有八个针状光束的PUV-PAM，以扩大景深，从而能够对表面不规则的无玻片组织进行快速成像。我们相信，PUV-PAM方法将实现快速的术中光声组织学成像，并为超快光学分辨率光声显微镜提供前景。
