Ma Ying, Ning Zhonghua
National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, PR China.
National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, PR China.
Poult Sci. 2025 Jan;104(1):104623. doi: 10.1016/j.psj.2024.104623. Epub 2024 Dec 5.
Eggshell translucency is the common problem on eggshell that become more severe with age. They are important because it influence consumer preferences and the economic value of eggs. The reason for the eggshell translucency is currently believed to be the eggshell membrane (ESM). In this study, we screened translucency eggs and normal eggs and used metabolomics to study liver metabolism in different eggshell translucency and discuss important liver lipids and phosphatidyl metabolites. Liver samples were taken for Liquid Chromatograph Mass Spectrometer (LC-MS) during the formation of eggshell membranes in hens, that is, when eggs form eggshell membranes in the oviduct isthmus. The results showed that we identified two essential metabolic pathways through differential metabolite pathway analysis, which were glycine, serine, and threonine metabolism related to amino acids metabolism and the PPAR metabolic pathway related to lipid metabolism. Furthermore, this study helps us understand the process of translucency egg production in poultry. This laid the foundation for in-depth research on eggshell translucency. These results may and provide support for future breeding.
蛋壳半透明是蛋壳上常见的问题，且随着鸡蛋存放时间变长而愈发严重。蛋壳半透明很重要，因为它会影响消费者的喜好以及鸡蛋的经济价值。目前认为蛋壳半透明的原因在于蛋壳膜（ESM）。在本研究中，我们筛选了半透明蛋和正常蛋，并利用代谢组学研究不同蛋壳半透明状态下的肝脏代谢，探讨重要的肝脏脂质和磷脂酰代谢产物。在母鸡形成蛋壳膜的过程中，即鸡蛋在输卵管峡部形成蛋壳膜时，采集肝脏样本用于液相色谱质谱联用仪（LC-MS）分析。结果表明，通过差异代谢物途径分析，我们确定了两条重要的代谢途径，一条是与氨基酸代谢相关的甘氨酸、丝氨酸和苏氨酸代谢途径，另一条是与脂质代谢相关的PPAR代谢途径。此外，本研究有助于我们了解家禽生产半透明蛋的过程。这为深入研究蛋壳半透明奠定了基础。这些结果可能为未来的育种提供支持。
