Luff R D, Kessler C M, Bell W R
Am J Clin Pathol. 1985 Jan;83(1):59-64. doi: 10.1093/ajcp/83.1.59.
Based on a continuing need to provide a more rapid response to requests for thawed fresh frozen plasma, the authors evaluated plasma thawing with the use of a microwave oven and compared it with conventional 37 degrees C waterbath thawing methods. Their results indicate that microwave-thawed plasma contains precipitated denatured protein (mainly albumin and fibrinogen) and that there is a significant reduction of coagulation Factors IX, X, XI, and fibrinogen compared with fresh plasma. They also measured levels of di-ethyl hexyl phthalate after microwave thawing and found its rate of accumulation similar to that of the 37 degrees C waterbath. More importantly, fundamental principles of microwave heating preclude uniform temperatures being maintained throughout the thawing of plasma; hence, the denaturation of plasma proteins is expected to occur under even low heating conditions.
基于对解冻新鲜冰冻血浆请求提供更快速响应的持续需求，作者评估了使用微波炉解冻血浆的方法，并将其与传统的37℃水浴解冻方法进行比较。他们的结果表明，微波解冻的血浆含有沉淀的变性蛋白质（主要是白蛋白和纤维蛋白原），并且与新鲜血浆相比，凝血因子IX、X、XI和纤维蛋白原显著减少。他们还测量了微波解冻后邻苯二甲酸二（2-乙基己基）酯的水平，发现其积累速率与37℃水浴相似。更重要的是，微波加热的基本原理使得在血浆解冻过程中无法保持均匀的温度；因此，即使在低加热条件下，血浆蛋白的变性也可能发生。
