Kraemer B B, Srigley J R, Batsakis J G, Silva E G, Goepfert H
Arch Otolaryngol. 1985 Jan;111(1):34-8. doi: 10.1001/archotol.1985.00800030068008.
Mechanically dispersed cell suspensions from 23 thyroid lesions were studied by acridine orange flow cytometry. Eight of 14 carcinomas (three papillary, two medullary, two Hürthle cell, and one follicular) manifested abnormal DNA indices ranging from 0.6 to 2.0. Six carcinomas (four papillary and two medullary) were diploid. Four patients having papillary carcinomas with diploid DNA content were young women whose clinical course of disease was indolent. Three papillary carcinomas with abnormal DNA content were found in older men with clinically aggressive disease. One benign adenomatoid nodule displayed a small population with a low-degree hyperdiploid stemline (DNA index = 1.1) with low proliferative activity. Differentiation between clinically indolent and aggressive carcinomas may be possible by nuclear DNA determination, but further work is needed to determine the importance of proliferative activity in thyroid carcinoma.
采用吖啶橙流式细胞术对23例甲状腺病变的机械分散细胞悬液进行了研究。14例癌中8例（3例乳头状癌、2例髓样癌、2例许特莱细胞癌和1例滤泡状癌）表现出异常的DNA指数，范围为0.6至2.0。6例癌（4例乳头状癌和2例髓样癌）为二倍体。4例DNA含量为二倍体的乳头状癌患者为年轻女性，其疾病临床进程缓慢。3例DNA含量异常的乳头状癌见于临床侵袭性疾病的老年男性。1个良性腺瘤样结节显示有一小群低增殖活性的低度超二倍体干系（DNA指数 = 1.1）。通过核DNA测定可能区分临床进程缓慢和侵袭性癌，但需要进一步研究以确定增殖活性在甲状腺癌中的重要性。
