Aliyyu Wienda Cinta, Riva Farahdika Ardelia, Anabel Sabrina Majesta Putri, Dwiandhono Irfan, Satrio Rinawati, Sari Dwi Nur Indah
Faculty of Medicine, Universitas Jenderal Soedirman. Banyumas, Central Java, Indonesia.
Dental and Oral Hospital of Universitas Jenderal Soedirman.
J Clin Exp Dent. 2024 Nov 1;16(11):e1323-e1331. doi: 10.4317/jced.62073. eCollection 2024 Nov.
This study aimed to compare rice snail shell nano-hydroxyapatite (nano-HAp) toothpaste combined with basil leaf extract and 1450 ppm fluoridated herbal toothpaste as remineralizing and antibacterial agents.
Experimental toothpastes were prepared with different concentrations of nano-HAp (5% nano-HAp, 10% nano-HAp) and a fixed concentration of basil leaf extract (5% extract). 24 bovine tooth enamel samples were divided into 4 treatment groups. After 6 days of pH cycling, surface microhardness was tested using Vickers Microhardness Test and microporosity was observed using Scanning Electron Microscopy (SEM). The pitting diffusion method was used to test the antibacterial activity of toothpaste against Streptococcus mutans and Lactobacillus acidophilus.
Enamel surface microhardness showed an increase with increasing nano-HAp concentration, while fluoride toothpaste showed the highest level of surface microhardness. Microporosity observation showed the disappearance of microporosity as nano-HAp concentration increased, while fluoride toothpaste still left microporosity. Antibacterial activity of the toothpaste showed that the mean inhibition zone of each group was not significantly different with a very strong inhibition zone.
Rice snail shell nano-HAp toothpaste combined with 5% basil leaf extract is effective as a remineralizing agent by reducing the level of tooth enamel microporosity but cannot match 1450 ppm fluoride toothpaste in increasing surface hardness. In addition, nano-hydroxyapatite toothpaste of paddy field snail shell combined with 5% basil leaf extract is effective as an antibacterial agent for preventing dental caries because it has antibacterial activity equivalent to 1450 ppm fluoride herbal toothpaste. Nano-hydroxyapatite, basil leaf extract, toothpaste, remineralization, antibacterial.
本研究旨在比较田螺壳纳米羟基磷灰石（纳米-HAp）牙膏与罗勒叶提取物以及1450 ppm含氟草本牙膏作为再矿化剂和抗菌剂的效果。
制备了含有不同浓度纳米-HAp（5%纳米-HAp、10%纳米-HAp）和固定浓度罗勒叶提取物（5%提取物）的实验牙膏。将24个牛牙釉质样本分为4个治疗组。经过6天的pH循环后，使用维氏显微硬度测试法测试表面显微硬度，并使用扫描电子显微镜（SEM）观察微孔率。采用点蚀扩散法测试牙膏对变形链球菌和嗜酸乳杆菌的抗菌活性。
牙釉质表面显微硬度随纳米-HAp浓度的增加而增加，而含氟牙膏的表面显微硬度最高。微孔率观察表明，随着纳米-HAp浓度的增加，微孔率消失，而含氟牙膏仍留有微孔率。牙膏的抗菌活性表明，每组的平均抑菌圈无显著差异，抑菌圈都很强。
田螺壳纳米-HAp牙膏与5%罗勒叶提取物结合作为再矿化剂可有效降低牙釉质微孔率水平，但在增加表面硬度方面无法与1450 ppm含氟牙膏相匹配。此外，田螺壳纳米羟基磷灰石牙膏与5%罗勒叶提取物结合作为预防龋齿的抗菌剂是有效的，因为它具有与1450 ppm含氟草本牙膏相当的抗菌活性。纳米羟基磷灰石、罗勒叶提取物、牙膏、再矿化、抗菌。
